运动训练对大鼠内毒素性肺损伤时热休克蛋白70表达的影响  

Effect of exercise training on heat shock protein 70 expression during endotoxin-induced acute lung injury in rats

作　　者：巩红岩[1] 郑芳[2] 胡晓慧 左志超 刘景景[1] 王庆志[3] 赵国安[1] Gong Hongyan;Zheng Fang;Hu Xiaohui;Zuo Zhichao;Liu Jingjing;Wang Qingzhi;Zhao Guoan(Department of Anesthesiology,First Affiliated Hospital of Xinxiang Medical University,Xinxiang 453000,China;Department of Ultrasound,First Affiliated Hospital of Xinxiang Medical University,Xinxiang 453000,China;School of Basic Medical Science,Xinxiang Medical University,Xinxiang 453000,China)

机构地区：[1]新乡医学院第一附属医院麻醉科,453000 [2]新乡医学院第一附属医院麻醉科超声科,453000 [3]新乡医学院基础医学院,453000

出　　处：《中华麻醉学杂志》2019年第5期637-640,共4页Chinese Journal of Anesthesiology

基　　金：河南省医学科技攻关计划联合共建项(2018020365)

摘　　要：目的评价运动训练对大鼠内毒素性肺损伤时热休克蛋白70(HSP70)表达的影响。方法SPF级健康雄性SD大鼠32只,8周龄,体重175~220 g。采用随机数字表法分为4组(n=8):对照组(C组)、肺损伤组(ALI组)、低强度运动训练组(ET1组)和高强度运动训练组(ET2组)。ET1组和ET2组大鼠于模型制备前分别进行2和4周的跑台运动训练,C组和ALI组大鼠不训练。随后ALI组、ET1组和ET2组大鼠通过尾静脉注射5 mg/kg LPS制备ALI模型,C组仅经尾静脉注射等量生理盐水。LPS或生理盐水注射后6 h,处死大鼠取肺组织,行肺组织病理学损伤评分,计算肺湿重/干重比值(W/D比值),测定肺泡灌洗液(BALF)总蛋白、IL-1β和TNF-α的浓度,采用Western blot法检测HSP70和NF-κB的表达。结果与C组比较,ALI组肺W/D比值和肺组织病理学损伤评分升高,BALF总蛋白、IL-1β和TNF-α的浓度升高,NF-κB表达上调(P<0.05),HSP70表达差异无统计学意义(P>0.05);与ALI组比较,ET1组和ET2组肺W/D比值和肺组织病理学损伤评分降低,BALF总蛋白、IL-1β和TNF-α的浓度降低,HSP70表达上调,NF-κB表达下调(P<0.05)。结论运动训练可通过缓解炎症反应减轻大鼠内毒素性肺损伤,可能与上调肺HSP70表达有关。Objective To evaluate the effect of exercise training on heat shock protein 70(HSP70)expression during endotoxin-induced acute lung injury(ALI)in rats.Methods Thirty-two SPF healthy male Sprague-Dawley rats,aged 8 weeks,weighing 175-220 g,were divided into 4 groups(n=8 each)using a random number table method:control group(group C),group ALI,low-intensity exercise training group(group ET1)and high-intensity exercise training group(group ET2).The rats in ET1 and ET2 groups received 2-and 4-week treadmill exercise training before establishing the ALI model,while the rats in C and ALI groups received no training.ALI was induced by intravenously injecting 5 mg/kg lipopolysaccharide via the tail vein in ALI,ET1 and ET2 groups,and the equal volume of normal saline was given instead in group C.The animals were sacrificed,and the lungs were harvested for microscopic examination of the pathological changes of lung tissues which were also scored and for determination of wet to dry weight ratio(W/D ratio),concentrations of total protein,interleukin-1beta(IL-1β)and tumor necrosis factor-alpha(TNF-α)in bronchoalveolar lavage fluid(BALF),and expression of HSP70 and nuclear factor kappa B(NF-κB)in lung tissues by Western blot.Results Compared with group C,the W/D ratio and pathological changes of lung tissues were significantly increased,the concentrations of total protein,IL-1βand TNF-αin BALF were increased,the expression of NF-κB was up-regulated(P<0.05),and no significant change was found in HSP70 expression in group ALI(P>0.05).Compared with group ALI,the W/D ratio and pathological changes of lung tissues were significantly decreased,the concentrations of total protein,IL-1βand TNF-αin BALF were decreased,the expression of HSP70 was up-regulated,and the expression of NF-κB was down-regulated in ET1 and ET2 groups(P<0.05).Conclusion Exercise training can attenuate the endotoxin-induced ALI through relieving the inflammatory responses,which may be related to up-regulating HSP70 expression in the lung of rats.

关 键 词：运动 内毒素血症 急性肺损伤 HSP70热休克蛋白质类 

分 类 号：R73[医药卫生—肿瘤]