Altering sub-cellular location for bioimaging by engineering the carbon based fluorescent nanoprobe  

通过碳基荧光纳米探针的设计调控其亚细胞定位和生物成像（英文）

作　　者：Chenchen Li Kangkang Zhang Chenjie Yao Lin Ding Yanan Huang Xuelian Yin Junfeng Zhang Wenchao Gao Jian Zhang Minghong Wu Yanli Wang 李晨晨;章康康;姚晨婕;丁琳;黄雅男;尹雪莲;章俊峰;高文超;张建;吴明红;王艳丽(Tumor Precision Targeting Research Center,School of Environmental and Chemical Engineering,Shanghai University,Shanghai 200444,China;Institution of Nanochemistry and Nanobiology,School of Environmental and Chemical Engineering,Shanghai University,Shanghai 200444,China;Universal Medical Imaging Diagnostic Research Center,Shanghai 200233,China;Harvard T.H.Chan School of Public Health,Harvard University,Boston,Massachusetts 02115,USA;Department of Biological Chemistry,The University of Michigan,Ann Arbor,MI 48109,USA)

机构地区：[1]Tumor Precision Targeting Research Center,School of Environmental and Chemical Engineering,Shanghai University,Shanghai 200444,China [2]Institution of Nanochemistry and Nanobiology,School of Environmental and Chemical Engineering,Shanghai University,Shanghai 200444,China [3]Universal Medical Imaging Diagnostic Research Center,Shanghai 200233,China [4]Harvard T.H.Chan School of Public Health,Harvard University,Boston,Massachusetts 02115,USA [5]Department of Biological Chemistry,The University of Michigan,Ann Arbor,MI 48109,USA

出　　处：《Science China Materials》2019年第10期1496-1504,共9页中国科学（材料科学（英文版）

基　　金：supported by the National Natural Science Foundation of China(21371115,11025526,1175107,21101104 and 11422542);Shanghai University-Universal Medical Imaging Diagnostic Research Foundation(19H00100);the Program for Changjiang Scholars and Innovative Research Team in University(IRT13078)

摘　　要：In recent years,numerous classes of carbon-based nanomaterials,such as carbon nanotubes(CNTs),carbon dots(CDs),graphene and its derivatives,graphene quantum dots(GQDs)and fullerene,have been deeply explored for potential applications in the biological fields,e.g.,bioimaging[1-5],biosensing[6,7],drug nanocarrier[8-12],etc.,owing to their unique and alluring physical and chemical properties.Among them,GQDs are a subject of interesting and promising research with many advantages such as strong signal strength,resistance to photobleaching,tunable fluorescence emissions,high sensitivity and biocompatibility[13-35].Compared with those semiconductor QDs,GQDs have remarkable superior让y in low toxicity,excellent biocompatibility,low cost,and abundance of original materials in nature[36].High quality GQDs have a wide range of applications,such as light emitting diodes(LEDs)[37,38],solar cells[39,40]and photocatalysis[41,42],aside from biological fields.Up to now,various GQDs with different photoluminescent(PL)colors have been synthesized by two dominating approaches including top-down and bottomup methods.The top-down method refers to cutting bulk carbon materials into nanoscale-carbon materials by necessary physical and chemical processes[43].碳基荧光纳米探针在生物成像领域展现出诱人的应用前景.本文通过调节合成方法合成了一种磺酸基修饰的石墨烯量子点(SGQDs)荧光探针.该探针呈现出优异的光学和理化性能,如荧光强度高、pH稳定、表面带负电等.研究表明其发光机理主要依赖荧光分子发光机制.与我们之前报道的氨基化量子点A-GQDs和肿瘤细胞核靶向探针GTTN对比,该探针具有良好的生物安全性,可以在相当短的时间内即跨膜进入细胞,而GTTN在正常的体外培养条件下无法进入细胞.为此,我们探究了产生该差异的原因.结果表明,S-GQDs与A-GQDs截然不同的合成原料导致了他们的毒性差异,而S-GQDs的不稳定性则是导致其进入细胞、与GTTN明显不同的主要原因.

关 键 词：肿瘤细胞核 石墨烯量子点 生物成像 合成原料 生物安全性 体外培养条件 荧光分子 发光机制 

分 类 号：R73[医药卫生—肿瘤]