病毒性心肌炎与人类主要组织相容性复合体多态性的相关性研究  

Association between MHC Gene Polymorphism and VMC

作　　者：刘嫚嫚 都鹏飞[1] LIU Manman;DU Pengfei(The second hospital of Anhui Medical University,Hefei 230000,China)

机构地区：[1]安徽医科大学第二附属医院儿科

出　　处：《实验动物科学》2019年第4期44-50,共7页Laboratory Animal Science

摘　　要：目的探索主要组织相容性复合体(major histocompatibility complex,MHC)等位基因与病毒性心肌炎(viral myocarditis,VMC)的关联性,以阐明VMC的免疫遗传学特征,并进一步探寻VMC发病中的保护基因,为可能的基因治疗提供靶基因探索新途径。方法创建急性病毒性心肌炎的小鼠模型,通过血清炎症因子IFN-γ水平、心肌组织病理改变及心肌组织MHC基因表达水平的不同反映出病毒性心肌炎与MHC多态性之间有无相关性。结果在32只病毒组小鼠中,MudoEb 5检测阳性的共有24只(基因频率37.5%),MudoEb 7检测阳性的有9只(基因频率14.1%),对照组中检测出MudoEb5阳性的有6只(基因频率9.4%),MudoEb7阳性共2只(基因频率3.1%),两种等位基因的表达与对照组相比差异均有统计学意义(MudoEb 5λ^2=20.33 P=0.00 OR=13,MudoEb 7,λ^2=5.38 P=0.02 OR=5.7)。结论MudoEb5、MudoEb7基因型表达水平与COXB3的感染小鼠的病理改变存在正相关,并根据统计学分析,两种等位基因型可能为VMC小鼠的易感基因,其中MudoEb5基因型与VMC的病理相关密切程度更高。Objective To investigate the correlations between MHC gene polymorphism and the development of viral myocarditis and to identify the susceptibility genes for VMC for further elucidating the pathogenesis of VMC and providing the guidance for researches on further gene therapy for VMC.Method Few genotypes of H2-Eb(including MudoEb 5、MudoEb7)in 32 VMC mice and 32 normal control group were detected by using polymerase chain reaction,as the same time,the serum level of IFN-γand the pathological changes of VMC mice were monitored to reflect the relationship between VMC and MHC gene polymorphism.Result The frequencies of H2-Eb MudoEb5/Eb7 in VMC mice(37.5%/9.4%)were higher than those in normal control group(14.1%/3.1%)(MudoEb 5λ^2=20.33 P=0.00 OR=13,MudoEb 7,λ^2=5.38 P=0.02 OR=5.7).Conclusion The genotypes MudoEb5、MudoEb7 might be the susceptibility genes for VMC in mice.

关 键 词：病毒性心肌炎 人类主要组织相容性复合 基因多态性 IFN-Γ 

分 类 号：R-332[医药卫生]