沉香提取液对乙醇致大鼠胃溃疡的保护作用及机制  

Protective effect and mechanism of Chinese eaglewood extract on gastric ulcer induced by ethanol in rats

作　　者：马麟[1] 张昕 陈焕鑫 周海玲[1] 李嘉琪 王羚郦 MA Lin;ZHANG Xin;CHEN Huanxin;ZHOU Hailing;LI Jiaqi;WANG Lingli(Department of Pharmacy,the Second Clinical Medical College,Guangzhou University of Chinese Medicine,Guangdong Province,Guangzhou510120,China;Mathematical Engineering Academy of Chinese Medicine,Guangzhou University of Chinese Medicine,Guangdong Province,Guangzhou510006,China)

机构地区：[1]广州中医药大学第二临床医学院药学部,广东广州510120 [2]广州中医药大学中医药数理工程研究院,广东广州510006

出　　处：《中国医药导报》2019年第24期15-18,共4页China Medical Herald

基　　金：广东省名优中成药二次开发项目(粤中医办函[2017]19号);广东省省级科技计划项目(2014B090902002);广东省协同创新与平台环境建设专项资金(东财函[2016]2250号)

摘　　要：目的研究沉香提取液对胃溃疡组织的保护作用及其作用机制。方法将60只SPF级的SD雄性大鼠按随机数字表法分为正常组(不予处理),模型组(等体积生理盐水),阳性对照组(兰索拉唑0.003 g/mL),沉香提取液低(0.4 g/mL)、中(0.8 g/mL)、高(1.6 g/mL)剂量组,给药剂量均为1 mL/100 g,每组10只。灌胃无水乙醇1 mL建立急性胃黏膜损伤模型,阳性对照组和沉香提取液各给药组每天灌胃给药1次,连续7 d。采用免疫组化检测大鼠胃组织中Bcl-2和Bax的含量。采用试剂盒检测大鼠血清中氧化因子微量丙二醛(MDA)的含量以及抗氧化因子超氧化物歧化酶(SOD)、微量还原性谷胱甘肽(GSH)的活性。结果与正常组比较,模型组胃组织Bcl-2蛋白表达下降,Bax蛋白表达上升(P<0.05)。与模型组比较,沉香提取液各给药组Bcl-2蛋白表达明显上升,Bax蛋白表达明显减少(P<0.05)。与模型组比较,沉香提取液各给药组能有效提高血清中SOD和GSH活性,降低MDA含量(P<0.05)。结论沉香提取液可能通过升高胃组织中Bcl-2蛋白表达、减少Bax蛋白表达、提高血清中SOD和GSH活性、降低MDA含量来改善无水乙醇致大鼠的胃溃疡。Objective To study the protective effect of Chinese eaglewood extract on gastric ulcer tissue and its mechanism.Methods Sixty SPF Sprague-Dawley(SD)male rats were divided into normal group(not to deal with),model group(equal volume of normal saline),positive control group(Lansoprazol 0.003 g/mL),Chinese eaglewood extract low(0.4 g/mL),medium(0.8 g/mL)and high(1.6 g/mL)dose groups by random number table method,the dosage of each group was 1 mL/100 g,with 10 rats in each group.The acute gastric mucosal injury model was established by intragastric administration of anhydrous ethanol 1 mL.The positive control group and Chinese eaglewood extract groups were given intragastric administration once a day for 7 days.The contents of Bcl-2 and Bax were examined by immunohistochemical method.The kit was used to detect the content of oxidative factor malondialdehyde(MDA)and the activity of antioxidant factor superoxide dismutase(SOD)and trace reduced glutathione(GSH)in rat serum.Results Compared with the normal group,the expression of Bcl-2 protein in the gastric tissue of the model group decreased,and the expression of Bax protein increased(P<0.05).Compared with the model group,the expression of Bcl-2 in the gastric tissue of Chinese eaglewood extract groups increased,and the expression of Bax protein decreased(P<0.05).Compared with the model group,the activity of SOD and GSH of Chinese eaglewood extract groups increased and the content of MDA decreased(P<0.05).Conclusion Chinese eaglewood extract can improve the gastric ulcer induced by absolute ethanol through increasing the expression of Bcl-2 protein in gastric tissue,reducing the expression of Bax protein,improving the activity of serum SOD and GSH and decreasing the content of MDA.

关 键 词：沉香 抗胃溃疡 免疫组化 作用机制 

分 类 号：R737.14[医药卫生—肿瘤]