siRNA沉默KLF4基因表达对人胶质瘤细胞增殖及凋亡的影响  

Effects of Inhibition of KLF4 Expression by siRNA on the Proliferation and Apoptosis of Human Glioma Cells

作　　者：郭春梅[1] 赵旭[1] GUO Chunmei;ZHAO Xu(Department of Neurosurgery,First Affiliated Hospital of Harbin Medical University,Harbin,Heilongjiang,150001,China)

机构地区：[1]哈尔滨医科大学附属第一医院神经外科

出　　处：《肿瘤药学》2019年第4期550-554,共5页Anti-Tumor Pharmacy

摘　　要：目的研究Krüppel样因子4(KLF4)对人脑胶质瘤细胞增殖与凋亡的影响。方法设计并合成干扰KLF4表达的小干扰RNA(siKLF4),转染人脑胶质瘤U251细胞,CCK8法检测细胞的增殖能力,流式细胞术检测细胞的凋亡率,Hoechst 33258染色法观察细胞凋亡情况,Western blotting法检测Caspase-3、Cleaved-Caspase-3、Caspase-9、Cleaved-Caspase-9的表达水平。结果siKLF4可有效抑制U251细胞的增殖并促进其凋亡。Western blotting结果显示,siKLF4可上调U251细胞中促凋亡信号分子Caspase-3、Cleaved-Caspase-3、Caspase-9、Cleaved-Caspase-9的表达水平。结论下调脑胶质瘤细胞中KLF4基因的表达有望为胶质瘤基因靶向治疗提供新的靶点。Objective To study the effects of inhibition of Krüppel-like factor 4(KLF4)gene expression on human glioma cells proliferation and apoptosis.Methods Small interference RNA(siKLF4)was designed and synthesized to interfere KLF4 expression,and transfected into human brain glioma U251 cells.CCK8 method was used to detect the cell proliferation.Flow cytometry was used to detect apoptosis rate of cells,and Hoechst 33258 dying was used to observe the cell apoptosis.Western blotting method was used to detect the expression of Caspase-3,Cleaved-Caspase-3,Caspase-9 and Cleaved-Caspase-9.Results siKLF4 could effectively inhibit the proliferation and promote the apoptosis of U251 cells.Western blotting results showed that siKLF4 up-regulated the expression of Caspase-3,Cleaved-Caspase-3,Caspase-9 and Cleaved-Caspase-9 in U251 cells.Conclusion Down-regulation of KLF4 gene expression is expected to provide a new target for glioma gene-targeted therapy.

关 键 词：Krüppel样因子4 基因 胶质瘤 细胞增殖 

分 类 号：R739.41[医药卫生—肿瘤]