载表阿霉素乙酰普鲁兰叶酸偶合体抗宫颈癌的作用研究  

Anti-tumor Effects and Mechanism Study of Epirubicin Loaded FolateTargeted Pullulan Acetate Nanoparticles

作　　者：唐红波[1,2] 冯欣[1] 王爱华[1] 胡海鹏 周密[1] 阴赪宏[1] 张其清[2] TANG Hongbo;FENG Xin;WANG Aihua;HU Haipeng;ZHOU Mi;YIN Chenghong;ZHANG Qiqing(Pharmacy Department,Beijing Obsterics and Gynecology Hospital,Capital Medical University,Beijing,100006,China;Institute of Biomedical Engineering,Chinese Academy of Medical Sciences/Peking Union Medical College,Tianjin,300192,China)

机构地区：[1]首都医科大学附属北京妇产医院药事部,北京100006 [2]中国医学科学院生物医学工程研究所,天津300192

出　　处：《肿瘤药学》2019年第4期555-559,共5页Anti-Tumor Pharmacy

基　　金：北京市医院管理局“登峰”计划专项（DFL20151301）;首都医科大学附属北京妇产医院中青年学科骨干培养专项（FCYY201707）

摘　　要：目的研究包载表阿霉素的乙酰普鲁兰叶酸偶合体纳米粒子(FPA/EPI)体内抗宫颈癌的作用及其机制。方法以人宫颈癌HeLa细胞移植瘤裸鼠为模型,分析尾静脉注射5 mg·kg^-1 FPA/EPI对荷瘤裸鼠体重、血液学、移植瘤体积和重量的影响,采用苏木精-伊红染色(HE)和原位末端标记法(TUNEL)观察FPA/EPI对移植瘤细胞凋亡的影响。结果FPA/EPI对HeLa细胞移植瘤裸鼠的体重未见明显影响,对肿瘤的生长具有抑制作用,肿瘤重量抑制率为32.37%。EPI及FPA/EPI均可诱导HeLa细胞凋亡。与对照组相比,FPA/EPI组网织红细胞显著降低(P<0.01)。结论FPA/EPI具有抗宫颈癌细胞移植瘤作用,但其药效和安全性尚需进一步的研究以明确。Objective To investigate the anti-cervical cancer effect and mechanism of epirubicin(EPI)loaded folate-targeted pullulan acetate(FPA)nanoparticles.Methods Based on the HeLa cells transplantation tumor model,the effects of 5 mg·kg^-1 FPA/EPI were investigated on the bodyweight,haematological variables,growth and weight of tumor tissues of the nude mice.The hematoxylin-eosin staining(HE)and terminal-deoxynucleotidyltransferase mediated nick end labeling method(TUNEL)were applied to observe the apoptosis of HeLa cells in the transplantation tumors.Results FPA/EPI showed no obvious adverse effects on the bodyweight of tumor-bearing nude mice.The tumor weight of FPA/EPI group was much less than that of control group,and the inhibition rate was 32.37%.EPI and FPA/EPI both could induce apoptosis of HeLa cells.Compared with control group,the reticulocyte of FPA/EPI group were reduced significantly(P<0.01).Conclusion FPA/EPI showed anticancer effects,but its efficacy and safety still need further research.

关 键 词：纳米粒子 乙酰普鲁兰 叶酸 宫颈癌 表阿霉素 

分 类 号：R737.33[医药卫生—肿瘤]