17-β雌二醇对子宫内膜上皮细胞转化的影响及其机制研究  

Investigation of the Effects of 17-β-estradiol on Endometrial Epithelial Cells Transformation and Its Signaling Mechanism in Cells

作　　者：王三猛 李文 王武亮[1] 赵虎[1] 张子规 闫莉莉 汪灿 于甜甜 WANG Sanmeng;LI Wen;WANG Wuliang;ZHAO Hu;ZHANG Zigui;YAN Lili;WANG Can;YU Tiantian(Department of Obstetrics and Gynecology,the Second Affiliated Hospital of Zhengzhou University,Zhengzhou,Henan,450014,China)

机构地区：[1]郑州大学第二附属医院妇产科

出　　处：《肿瘤药学》2019年第4期560-564,共5页Anti-Tumor Pharmacy

摘　　要：目的探究17-β雌二醇(E2)对子宫内膜上皮细胞(EEC)转化的影响及其机制。方法以G蛋白偶联雌激素受体(GPER)阳性、雌激素受体(ER)阴性子宫内膜上皮细胞为模型细胞,E2连续刺激11周,适时加入G15进行预处理,显微镜下观察细胞形态变化,MTT法检测细胞增殖能力,蛋白印迹法检测蛋白表达情况,软琼脂克隆实验检测细胞非贴壁依赖克隆形成能力。结果11周后,E2处理后模型细胞的细胞接触抑制消失、胞体变大、呈梭形生长。设DMSO组细胞增殖率、克隆形成率和GPER蛋白表达量均为100%,则E2组48 h时细胞增殖率为(138.6±9.6)%,克隆形成率为(252.4±13.6)%,GPER蛋白表达量为(210.7±15.2)%,与DMSO组相比,差异均有统计学意义(P<0.01)。与E2组相比,E2+G15组细胞接触抑制,胞体较小,呈纤维形生长。48 h时,E2组细胞增殖率为(138.6±9.6)%,E2+G15组为(101.8±8.2)%,差异有统计学意义(P<0.05)。设E2组细胞克隆形成率为100%,则E2+G15组为(64.3±6.2)%,差异有统计学意义(P<0.05)。设E2组GPER蛋白表达水平为100%,则E2+G15组为(36.0±3.1)%,差异有统计学意义(P<0.05)。结论E2可诱导EEC转化,其机制可能与GPER有关。Objective To observe the effects of long-term over-stimulation of 17-βestradiol(E2)on the transformation of endometrium epithelial cells(EEC),and to study its signaling mechanism.Methods The G protein-coupled estrogen receptor(GPER)-positive and estrogen receptor(ER)-negative endometrial epithelial EECs were used as model cells.Cells were designed into three groups.The control group was treated with dimethylsulfoxide,also called DMSO group.E2 group was treated with 50 nmol·mL-1 E2.And E2+G15 group got treatment of 10μmol·mL-1 G15 before E2 stimulation.The microscope was used to observe the cell morphological changes.MTT assay was applied to detect cell proliferation.Western blotting was applied to detect protein expression changes,and soft agar clone assay was to detect anchorage-independent cell colony formation capabilities.Results After E2 stimulated the model cell for 11 weeks,the cell contact inhibition disappeared,the cell body became larger,and it showed spindle-shaped growth.At 48 h,the cell proliferation rate of DMSO group was 100%,and that of E2 group was(138.6±9.6)%,the difference was significant(P<0.01).The colony formation rate of DMSO group was 100%,and that of E2 group was(252.4±13.6)%,and the difference was significant(P<0.01).The relative protein expression level of GPER in DMSO group was 100%,and that in E2 group was(210.7±15.2)%,and the difference was significant(P<0.01).Compared with E2 group,the cells in E2+G15 group were inhibited by contact,and the cell bodies were small and fibrous.At 48 h,the cell proliferation rate of E2 group was(138.6±9.6)%,and that of E2+G15 group was(101.8±8.2)%,the difference was statistically significant(P<0.05).The colony formation rate of E2 group was 100%,and that of E2+G15 group was(64.3±6.2)%,and the difference was significant(P<0.05).The relative protein expression level of GPER in E2 group was 100%, and that in E2+G15 group was (36.0±3.1) %, and the difference was significant(P<0.05). Conclusion E2 may induce EEC transformation, and such effect ma

关 键 词：17-Β雌二醇 子宫内膜上皮细胞 G蛋白偶联雌激素受体 转化 

分 类 号：R737.33[医药卫生—肿瘤]