基于PPARγ、PTEN基因探讨胃复方含药血浆对人胃癌BGC-823细胞的影响  

Effects of Weifufang-Medicated Plasma on Human Gastric Cancer BGC-823 Cells Based on PPARγ and PTEN Gene

作　　者：李东芳[1] 李超年 张顺荣 何寄琴 焦蕉[1] 唐继云 何欣[1] 周珉[1] LI Dongfang;LI Chaonian;ZHANG Shunrong;HE Jiqin;JIAO Jiao;TANG Jiyun;HE Xin;ZHOU Min(Hunan Cancer Hospital,Changsha,Hunan,410013,China;Binhai People's Hospital,Yancheng,Jiangsu,224500,China)

机构地区：[1]湖南省肿瘤医院,湖南长沙410006 [2]江苏省滨海县人民医院,江苏盐城224500

出　　处：《肿瘤药学》2019年第4期565-571,共7页Anti-Tumor Pharmacy

基　　金：湖南省自然科学基金面上项目（2017JJ2174）;湖南省中医药管理局重点项目（201719）;《黎月恒全国名老中医药专家传承工作室》湘财社【2014】119号

摘　　要：目的观察胃复方含药血浆对人胃癌BGC-823细胞凋亡及PPARγ、PTEN表达的影响,并探讨其作用机制。方法通过灌胃给药制备胃复方含药血浆,采用MTS法检测胃复方含药血浆对人胃癌BGC-823细胞生长的影响,并确定后续给药浓度。将体外培养的人胃癌BGC-823细胞随机分为胃复方含药血浆组(15%胃复方含药血浆)、空白对照组(15%磷酸缓冲盐溶液)、阳性对照组(15%氟尿嘧啶含药血浆)、阴性对照组(15%蒸馏水血浆),处理72 h后采用流式细胞术检测细胞凋亡,采用RT-PCR法、蛋白质印迹法分别检测PPARγ、PTEN mRNA或蛋白的表达水平。结果与空白对照组比较,胃复方含药血浆组与阳性对照组的细胞凋亡率、PPARγmRNA及蛋白表达水平、PTEN mRNA及蛋白表达水平均显著升高(P<0.05);与阴性对照组比较,胃复方含药血浆组与阳性对照组的细胞凋亡率、PPARγmRNA及蛋白表达水平、PTEN mRNA及蛋白表达水平均显著升高(P<0.05);但胃复方含药血浆组的细胞凋亡率、PPARγmRNA及蛋白表达水平、PTEN mRNA及蛋白表达水平均显著低于阳性对照组(P<0.05)。结论胃复方含药血浆可诱导人胃癌BGC-823细胞凋亡,其作用机制可能与活化的PPARγ上调PTEN表达有关。Objective To observe the effect of Weifufang-medicated plasma on apoptosis of human gastric cancer BGC-823 cells and expressions of PPARγ and PTEN, and to explore the mechanism of its effect on gastric cancer. Methods Weifufang-medicated plasma was prepared through gavage. MTS was used to detect the effect of Weifufang-medicated plasma on the growth of human gastric cancer BGC-823 cells, and the concentration of subsequent experiments was determined. BGC-823 cells cultured in vitro were randomly divided into three groups: Weifufang-medicated plasma group(15% drug containing plasma), blank control group(15% phosphate buffer salt solution), positive control group(15% fluorouracil containing plasma), and negative control group(15% distilled water containing plasma). After 72 hours of treatment, the apoptosis of BGC-823 cells was detected by flow cytometry, and the mRNA or protein expressions of PPARγ and PTEN were detected by RT-PCR assay or Western blotting. Results Compared with the blank control group, the apoptotic rate of BGC-823 cells, the expressions of PPARγ mRNA and protein, PTEN mRNA and protein were increased in the Weifufang-medicated plasma group and the positive control group(P<0.05). Compared with the negative control group, the apoptotic rate of BGC-823 cells, expressions of PPARγ and PTEN in both mRNA and protein level were increased in the Weifufang-medicated plasma group and the positive control group(P<0.05). However, the apoptotic rate of BGC-823 cells, and both mRNA and protein expression levels of PPARγ and PTEN were lower in Weifufang-medicated plasma group than in positive control group(P<0.05). Conclusion Weifufang-medicated plasma can induce apoptosis of human gastric cancer BGC-823 cells, and its mechanism may be related to the up-regulation of PTEN expression by activated PPAR γ.

关 键 词：胃复方 含药血浆 PPARΓ PTEN BGC-823细胞 细胞凋亡 

分 类 号：R735.2[医药卫生—肿瘤]