Molecular Cloning,Genomic Organization and Functional Analysis of the Ribosomal Protein S30(RPS30) Gene from Arachis hypogaea  

作　　者：WU Qi WANG Xiuzhen TANG Yueyi WANG Zhiwei WANG Chuantang 吴琪;王秀贞;唐月异;王志伟;王传堂(Shandong Peanut Research Institute (SPRI))

机构地区：[1]Shandong Peanut Research Institute (SPRI)

出　　处：《Journal of Donghua University(English Edition)》2019年第3期267-276,共10页东华大学学报（英文版）

基　　金：Natural Science Funds for Young Scholar of Shandong Academy of Agricultural Sciences,China(No.2015YQN13);Natural Science Foundation of Shandong Province,China(ZR2015YL064);Qingdao Science and Technology Plan Basic Research Project,China(No.12-1-4-11-(1)-jch);China Agricultural Research System(No.CARS-13);Agricultural Scientific and Technological Innovation Project of Shandong Academy of Agricultural Sciences,China(No.CXGC2018E21)

摘　　要：The ribosomal proteins are crucial for the maintenance of ribosomal translational efficiency and fidelity.In the study,we characterized the ribosomal protein S30(RPS30)gene from Arachis hypogaea that has been isolated through Genefishing analysis during defense responses to Ralstonia solanacearum.The cDNA of RPS 30 contained a 189 base pair(bp)open-reading frame encoding 62 amino acids.The genomic DNA consists of 272 bp containing two exons and one 83 bp intron.The RPS 30 mRNA transcript was mainly expressed in roots and leaves.The expression level of the RPS 30 mRNA transcripts was up-regulated sharply 6 h after bacterial challenge and was 12 times greater than that of the control group.The phylogenetic analysis for genes encoding proteins showed that RPS30 were conserved within dicotyledonous and monocotyledonous plants.d S extremely exceeded d N in all branches of the tree(d N/d S<1.0),indicating that functional constraint have acted on RPS 30 throughout evolution.The ribosomal proteins are crucial for the maintenance of ribosomal translational efficiency and fidelity. In the study, we characterized the ribosomal protein S30(RPS30) gene from Arachis hypogaea that has been isolated through Genefishing analysis during defense responses to Ralstonia solanacearum. The cDNA of RPS30 contained a 189 base pair(bp) open-reading frame encoding 62 amino acids. The genomic DNA consists of 272 bp containing two exons and one 83 bp intron. The RPS30 mRNA transcript was mainly expressed in roots and leaves. The expression level of the RPS30 mRNA transcripts was up-regulated sharply 6 h after bacterial challenge and was 12 times greater than that of the control group. The phylogenetic analysis for genes encoding proteins showed that RPS30 were conserved within dicotyledonous and monocotyledonous plants. d_S extremely exceeded d_N in all branches of the tree(d_N/d_S < 1.0), indicating that functional constraint have acted on RPS30 throughout evolution.

关 键 词：RIBOSOMAL protein S30(RPS30) ARACHIS HYPOGAEA Ri Hua 1 PURIFYING selection 

分 类 号：S184[农业科学—农业基础科学]