牦牛骨抗氧化肽的分离纯化及鉴定  

Isolation, Purification and Identification Antioxidant Peptides from Yak Bone

作　　者：姜海洋[1] 张周莉 孙敏 王境 杨宽[1] 唐诗宇 李霞雪 李姗姗[1] 周兰[1] 刘爱平[1] 李诚[1] Jiang Haiyang;Zhang Zhouli;Sun Min;Wang Jing;Yang Kuan;Tang Shiyu;Li Xiaxue;Li Shanshan;Zhou Lan;Liu Aiping;Li Cheng(Sichuan Agricultural University,Ya'an,625014)

机构地区：[1]四川农业大学

出　　处：《基因组学与应用生物学》2019年第8期3479-3485,共7页Genomics and Applied Biology

摘　　要：为了分离纯化及鉴定牦牛骨胶原多肽,以得到具有DPPH自由基清除率的抗氧化肽。合成抗氧化肽,验证其对DPPH自由基清除率。牦牛骨胶原多肽经陶瓷羟基磷灰石柱层析和C18反相高效液相色谱层析后得到组分F11。当其质量浓度为1 mg/mL时,对DPPH自由基清除率为34.45%。用反相高效液相色谱-质谱联用对F11组分进行序列测定,得到氨基酸序列为GPAGPPGPIGNVGAPGPK和GKSGDRGETGPAGP AGPIGPVGAR的抗氧化肽,分子量分别为1 570.810 3 Da和2 160.104 Da。合成抗氧化多肽GPAGPPGPIGN VGAPGPK和GKSGDRGETGPAGPAGPIGPVGAR,当其质量浓度为1 mg/mL时,对DPPH自由基清除率分别为16.59%和35.17%。本研究分离鉴定出牦牛骨抗氧化肽,牦牛骨可能成为一种潜在的抗氧化剂。In order to obtain antioxidant peptides which have DPPH radical scavenging rates, Yak bone collagen peptides were isolated, purified and identified. Synthesized peptides to verify the hydrolyzed peptides existing DPPH radical scavenging rates activities. One antioxidant peptide fraction, F11, which hydrolyzed from Yak bone collagen was isolated by ceramic hydroxyapatite chromatograpy and C18 RP-HPLC chromatograpy. When the concentration of F11 was 1 mg/mL, the DPPH radical scavenging rates of F11 was 34.45%. The components of F11 fraction were identified by RP-HPLC-MS/MS. Two peptides were detected, which the amino acid sequences were GPAGPPGPIGNVGAPGPK with molecular weight of 1 570.810 3 Da and GKSGDRGETGPAGPAGPIGPVGAR with molecular weight of 2 160.104 Da, respectively. When the concentrations of synthesized peptides were 1 mg/m L,the DPPH radical scavenging rates of GPAGPPGPIGNVGAPGPK and GKSGDRGETGPAGPAGPIGPVGAR were 16.59% and 35.17%, respectively. Antioxidant peptides were identified from Yak bone, which could be a potantial antioxidant.

关 键 词：酶解 陶瓷羟基磷灰石 反相高效液相 液质联用 DPPH自由基 

分 类 号：R28[医药卫生—中药学]