小檗碱对急淋白血病的在体抗肿瘤及与Ara-C的联合作用研究  

The Anti-Tumor Effect of Berberine on Acute Lymphoblastic Leukemia in vivo and the Combination Effect with Ara-C

作　　者：王成艳 何宏星 吴香新 施晓柯 Wang Chengyan;He Hongxing;Wu Xiangxin;Shi Xiaoke(Laboratory Animal Center,Fujian Medical University,Fuzhou,350108;The First Affiliated Hospital of Guangzhou Medical University,Guangzhou,510120)

机构地区：[1]福建医科大学实验动物中心,福州350122 [2]广州医科大学附属第一医院,广州510120

出　　处：《基因组学与应用生物学》2019年第8期3810-3814,共5页Genomics and Applied Biology

基　　金：福建省科技厅科技计划项目(No:2014Y0077)资助

摘　　要：本实验旨在研究小檗碱(berberine, BBR)对急淋白血病细胞Jurkat的在体抗肿瘤作用及与阿糖胞苷(cytosine, Ara-C)的联合作用。本研究通过体外培养Jurkat细胞株,将细胞注射至裸鼠皮下,建立皮下移植瘤模型,待肿瘤体积长至约100 mm^3时,随机分为两组:对照组和小檗碱处理组,分别口服PBS、200 mg/kg小檗碱,隔天给药一次,隔天记录裸鼠体重及肿瘤体积大小。给药30 d后处死动物,剥离肿瘤组织,称量肿瘤大小,绘制肿瘤生长曲线、体重图及瘤重图,计算抑瘤率;采用免疫组化法检测移植瘤组织中细胞核增殖抗原Ki-67的表达水平。同时,本研究用一线用药阿糖胞苷(cytarabine, Ara-C)与小檗碱联合处理Jurkat细胞,研究两药联用的药效。发现小檗碱处理组移植瘤大小受到明显的抑制,瘤块组织中Ki-67的表达明显降低;Ara-C与小檗碱能协同抑制Jurkat细胞的增殖,联合指数(combination index, CI)<1。本研究发现小檗碱能够抑制Jurkat细胞皮下移植瘤的增殖,在体内发挥抗急淋白血病活性,并能与一线用药Ara-C协同产生作用。To investigate the anti-cancer effect of berberine(BBR)on acute lymphoblastic Leukemia Jurkat cell in vivo and the combination effect with Ara-C,Jurkat cell was collected and injected into BalB/c nu/nu to establish xenograft model.When the tumor volume was approximately 100 mm3,Jurkat xenografts were randomized to treatment with PBS(control)or BBR(200 mg/kg/d p.o.)for 30 days.The weight of all the mice and tumor volume were measured and recorded every other day.Weight graph and tumor volume graph were drawed by GraphPad Prism 5.0 software.The Ki-67 levels of tumors were measured by immunohistochemical technology.Simultaneously,the combination between BBR and the frontline chemotherapeutic agents for ALL(e.g.,Ara-C)was tested by MTS assay.The results showed that BBR potently inhibited the growth of the tumors and the expression of K.i-67 was down-regulated.Furthermore,Ara-C and BBR synergistically(combination index(CI)<1)inhibited the viability of Jurkat cell.BBR displayed the anti-tumor effect in vivo by inhibiting the proliferation of Jurkat xenografts and was synergistical with Ara-C.

关 键 词：小檗碱 JURKAT细胞 皮下瘤 增殖 联合用药 

分 类 号：R73[医药卫生—肿瘤]