Hybrid polycaprolactone/hydrogel scaffold fabrication and in-process plasma treatment using PABS  

作　　者：Fengyuan Liu Hussein Mishbak Paulo Bartolo 

机构地区：[1]Department of Science and Engineering,School of Mechanical,Aerospace and Civil Engineering,University ofManchester,Manchester,M13 9PL,UK [2]Department of Biomedical Engineering,School of Engineering,University of Thi-Qar,Tai-Qar,Iraq

出　　处：《International Journal of Bioprinting》2019年第1期24-32,共9页国际生物打印期刊

摘　　要：A challenge for tissue engineering is to produce synthetic scaffolds of adequate chemical,physical,and biological cues effectively.Due to the hydrophobicity of the commonly used synthetic polymers,the printed scaffolds are limited in cell-seeding and proliferation efficiency.Furthermore,non-uniform cell distribution along the scaffolds with rare cell attachment in the core region is a common problem.There are no available commercial systems able to produce multi-type material and gradient scaffolds which could mimic the nature tissues.This paper describes a plasma-assisted bio-extrusion system(PABS)to overcome the above limitations and capable of producing functional-gradient scaffolds;it comprises pressure-assisted and screw-assisted extruders and plasma jets.A hybrid scaffold consisting of synthetic biopolymer and natural hybrid hydrogel alginate-gelatin(Alg-Gel)methacrylate anhydride,and full-layer N2 plasma modification scaffolds were produced using PABS.Water contact angle and in vitro biological tests confirm that the plasma modification alters the hydrophilicity properties of synthetic polymers and promotes proliferation of cells,leading to homogeneous cell colonization.The results confirm the printing capability for soft hard material integration of PABS and suggest that it is promising for producing functional gradient scaffolds of biomaterials.

关 键 词：Tissue engineering HYBRID SCAFFOLD PABS in-process plasma modification functional gradient SCAFFOLD 

分 类 号：R31[医药卫生—基础医学]