灰盖鬼伞原基发育过程中的蛋白质组变化  

Proteomes in Primordia Development of Coprinopsis cinerea

作　　者：MAJCHERCZYK Andrzej D?RNTE Bastian SUBBA Shanta ZOMORRODI Mojtaba KüES Ursula MAJCHERCZYK Andrzej;DORNTE Bastian;SUBBA Shanta;ZOMORRODI Mojtaba;KüES Ursula(Department of Molecular Wood Biotechnology and Technical Mycology,Büsgen-Institute and Goettingen Center for Molecular Biosciences(GZMB),University of Goettingen,Büsgenweg 37077 Gottingen,Germany)

机构地区：[1]树木分子生物技术与菌物技术系布森学院及哥廷根分子生物科学中心哥廷根大学布森

出　　处：《食用菌学报》2019年第3期37-50,共14页Acta Edulis Fungi

基　　金：German Science Foundation(DFG No.24946052)~~

摘　　要：灰盖鬼伞在25℃以及12 h光照/12h黑暗的环境下形成子实体。子实体最初在黑暗环境下通过大量生产菌丝分支形成初级菌丝结。光照信号触发初级菌丝结开始形成紧密的二级菌丝结。在随后原基发育的5 d中,菌盖和菌柄开始按照不同的光调控模式从这些菌丝结中分化,然后在第6天成熟。为了了解菌盖和菌柄组织形成时复杂的细胞过程,提取了原基各期的蛋白质组进行MS/MS质谱分析。结果显示,在不同的样本中检测到大约1672~2663个至少具有2个肽的蛋白,其中1401个蛋白是所有样本共有的,其中包含已知的涉及原基发育的蛋白。Fruiting body formation of Coprinopsis cinerea takes place at 25℃under a 12h day/12 h night regime.It starts by intense local hyphal branching with production of primary hyphal knots in the dark.A first light signal induces the transfer into the compact secondary hyphal knots.In these secondary hyphal knots,cap and stipe tissue begin to differentiate.This process underlies distinct patterns of light-and dark-regulated events over the following 5 days and then the mushrooms mature on the next day.To gain insight into the complex cytological processes during the cap and stipe tissue development,we isolated total proteomes from distinct primordia stages for MS/MS analysis.Between 1672 and 2663 proteins were detected in the different samples with at least two peptides at a confidence level of 99%,among which 1401 proteins were shared by all the samples.Known proteins in primordia development were identified in the samples.

关 键 词：菌丝结 原基 子实体 蛋白质功能 蛋白质组 质谱 

分 类 号：S64[农业科学—蔬菜学]