花溪古茶树离体繁殖技术研究初报  

Preliminary Report on in vitro Propagation Technology of Ancient Tea Tree in Huaxi District

作　　者：陈立杰[1] 杨霞 高倩 李悦 陈红[2] CHEN Lijie;YANG Xia;GAO Qian;LI Yue;CHEN Hong(School-run General Industrial Corporation,Guizhou University,Guiyang 550025,China;College of Agriculture,Guizhou University,Guiyang 550025,China;College of Science and Technology,Guizhou University,Guiyang 550025,China)

机构地区：[1]贵州大学校办产业总公司,贵州贵阳550025 [2]贵州大学农学院,贵州贵阳550025 [3]贵州大学科技学院,贵州贵阳550025

出　　处：《种业导刊》2019年第8期10-13,共4页Journal of Seed Industry Guide

摘　　要：为了建立花溪古茶树的组培快繁体系,以花溪古茶树单株为试验材料,对其带芽茎段最佳灭菌时间、腋芽启动、增殖及生根培养条件进行研究。结果表明,最佳灭菌时间为0.1%HgCl2消毒6 min;最有利于腋芽启动和增殖的培养基是MS+6-BA 1.5 mg/L+NAA 0.5 mg/L,所得最佳增殖系数高达1.5;适宜的生根培养基为1/2 MS+1.0 mg/L IBA+0.5 mg/L NAA,所得生根率为56.7%。In order to establish tissue culture and rapid propagation system of ancient tea tree in Huaxi district.Ancient tea tree in Huaxi district was used as test material,and the optimum sterilization time,axillary bud initiation,proliferation and rooting culture conditions of shoot segments with buds were studied.The results showed that the optimal sterilization time was 0.1%HgCl2 for 6 min.MS+6-BA 1.5 mg/L+NAA 0.5 mg/L was the best medium for axillary bud initiation and proliferation,and the optimal multiplication coefficient was 1.5.The suitable rooting medium was 1/2 MS+1.0 mg/L IBA+0.5 mg/L NAA,and the rooting rate was 56.7%.

关 键 词：花溪古茶树 茎段 离体繁殖 

分 类 号：S571.1[农业科学—茶叶生产加工]