水/正己烷双相体系全细胞合成D-苯基丙酸的研究  

Synthesis D-phenylpropionic Acid in Normal Hexane-Water Bipolar System

作　　者：陆炀 庞敬权 LU Yang;PANG Jing-quan(College of Food Science and Engineering,Yangzhou University,Yangzhou 225000,Jiangsu,China;Changshu Institute of Technology,Changshu 215500,Jiangsu,China)

机构地区：[1]扬州大学食品科学与工程学院,江苏扬州225000 [2]常熟理工学院,江苏常熟215500

出　　处：《江苏调味副食品》2019年第3期30-33,共4页Jiangsu Condiment and Subsidiary Food

摘　　要：利用前期筛选出的耐受苯基乳酸的突变菌株E.coli Z2016(CCTCC保藏编号为M2016332),在水相和有机溶剂正己烷组成的双相体系中转化生产苯基乳酸。实验还研究了转化温度、摇床转速、底物浓度、湿菌体浓度和有机溶剂正己烷的体积分数对PLA产量的影响。当转化温度为40℃、摇床转速为300 r/min、底物浓度和耐受PLA突变菌株E.coli Z2016菌体浓度均为15 g/L、正己烷的体积分数为40%的时候,PLA的产量最大,为10.9±0.08 g/L/h。This experiment used E.coli Z2016(the number of CCTCC is M2016332)which selected in the laboratory synthesize D-phenylpropionic acid in nomal hexane-water bipolar system.The experiment also studied the effect of volume fraction of n-hexane,concentration substrate,conversion speed,conversion temperature and E.coli Z2016 cell concentration on yield of PLA.When volume fraction of n-hexane was 40%,concentration substrate was 15 g/L,conversion speed was 300 r/min,conversion temperature was 40℃,E.coli Z2016 cell concentration was 15 g/L,the production of PLA was the most,10.9±0.08 g/L/h.

关 键 词：苯基乳酸 食品防腐剂 苯丙酮酸 双相体系 产率 

分 类 号：TS201[轻工技术与工程—食品科学]