一个复方中药处方对流感病毒A/PR/8/H1N1感染小鼠肺损伤的影响  

Effects of a compound Chinese medicine prescription on mouse lung injury induced by influenza virus A/PR/8/H1N1

作　　者：王学文 朱祥宇 李永清[3] WANG Xuewen;ZHU Xiangyu;LI Yongqing(Institute for Laboratory Animal Resources,National Institutes for Food and Drug Control,Beijing 102629,China;Experimental Animal Laboratory,Peking University People’s Hospital,Beijing 100044;Institute of Animal Husbandry and Veterinary Medicine,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097)

机构地区：[1]中国食品药品检定研究院实验动物资源研究所,北京102629 [2]北京大学人民医院动物实验室,北京100044 [3]北京市农林科学院畜牧兽医研究所,北京100097

出　　处：《中国比较医学杂志》2019年第9期75-80,共6页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金国际(地区)合作与交流项目(31761133003);北京市农林科学院科技创新能力建设专项(KJCX201914)

摘　　要：目的观察一个复方中药处方对流感病毒A/PR/8/H1N1感染小鼠所致肺损伤的影响。方法将90只雌性BALB/c小鼠随机分为空白对照组、病毒对照组、盐酸金刚烷胺组和复方中药高、中、低剂量组等6组,每组15只。中药组连续灌胃2 d后,小鼠滴鼻感染建立流感病毒小鼠模型,空白对照组滴鼻生理盐水。病毒感染4 h后各组分别灌胃不同剂量药物或生理盐水,再连续灌胃5 d。感染第5天时,计算各组BALB/c小鼠肺指数,测定肺组织匀浆的血凝滴度,并观察肺组织病理形态学。结果与病毒对照组相比,盐酸金刚烷胺组和复方中药制剂中剂量组肺指数显著降低(P<0.01),复方中药制剂低剂量组和复方中药制剂高剂量组肺指数显著降低(P<0.05);各药物组小鼠肺组织血凝滴度均显著降低(P<0.01);各药物组小鼠肺组织病理形态学均有不同程度的改善。结论本研究所用的复方中药处方对流感病毒A/PR/8/H1N1感染的小鼠平均肺指数和平均血凝滴度有明显的降低作用,对其所致肺损伤有明显的改善作用。Objective To examine the effect of a compound Chinese medicine prescription(provided by the Beijing Hongtianli pharmaceuticals)on the lung injury induced by influenza virus A/PR/8/H1N1 in mice.Methods Ninety female BALB/c mice were randomly divided into six groups:normal control group,virus control group,Amantadine hydrochloride group,and compound Chinese herbal medicine high dose group,medium dose group and low dose group(n=15 in each group).After intragastric administration of the drugs for 2 days,the infection model of influenza virus A/PR/8/H1N1 was established in mice by an intranasal challenge;the normal control group was injected with saline.Four hours later,each group was intragastrically administrated with different doses of medicine or saline,which were continuously given once daily for 5 days.The lung index of BALB/c mice in each group was calculated on the 5th day of infection,the hemagglutination titer of lung tissue homogenate was measured,and the pathological changes of the lung tissue was observed.Results Compared with the virus control group,the lung index in the Amantadine hydrochloride group and the medium dose compound Chinese medicine dose group was significantly decreased(P<0.01).This index was also decreased in the low and high dose herbal compound groups(P<0.05).The lung tissue viral hemagglutination titer was significantly decreased(P<0.01)in mice of all drug groups.The pathological changes in lung tissues in mice of all drug groups to a different degree.Conclusions The compound Chinese medicine prescription used in this study decreases the mean lung index and mean hemagglutination titer,and also improve the repair of lung injury in mice infected with influenza A/PR/8/H1N1.

关 键 词：复方中药 流感病毒A/PR/8/H1N1 肺损伤 小鼠 

分 类 号：R-33[医药卫生]