鸡源大肠杆菌分离鉴定及ESBLs与AmpC酶基因型检测及耐药性分析  

Isolation,Identification,Detection of ESBLs and AmpC Enzyme Genotypes and Drug Resistance Analysis of Escherichia coli from Chicken

作　　者：马馨[1] 刘一飞[1] 王志宇[1] 任彬琳 陈泽[1] 杜彬[1] 张菁[1] MA Xin;LIU Yifei;WANG Zhiyu;REN Binlin;CHEN Ze;DU Bin;ZHANG Jing(Institute of Animal Husbandry and Veterinary Science,Shanxi Academy of Agricultural Science,Taiyuan 030032,China)

机构地区：[1]山西省农业科学院畜牧兽医研究所

出　　处：《中国畜牧兽医》2019年第9期2715-2725,共11页China Animal Husbandry & Veterinary Medicine

基　　金：山西省农业科学院攻关项目(YGG1427)

摘　　要：为了解山西省范围内鸡源产超广谱β-内酰胺酶(ESBLs)和头孢菌素酶(AmpC)大肠杆菌分离株的基因型及其耐药现状,本试验从呈现典型鸡大肠杆菌病临床症状的病料中分离、鉴定出68株符合鸡源大肠杆菌生物特性的流行菌株,采用K-B法与双纸片确认法对分离的68株大肠杆菌分别进行药敏试验和ESBLs、AmpC酶耐药表型的筛选;采用PCR方法检测了分离株中质粒介导的ESBLs、AmpC酶的基因型。结果显示,分离到的68株菌对22种抗菌药物均产生不同程度的耐药性,其中耐药谱达7耐以上的有66,占总分离菌株数的97.06%;呈ESBLs、AmpC酶阳性和两者均阳性的菌株数分别为57(83.82%)、19(27.94%)和16(23.53%)株;检测出ESBLs阳性菌株携带的耐药基因型包括bla TEM、bla OXA和bla CTX-M-1/9,AmpC酶阳性菌株耐药基因型为bla FOX型。研究结果表明,分离的68株鸡源大肠杆菌已对绝大多数种类抗菌药物产生耐药性,且产ESBLs和AmpC酶菌株已普遍流行。山西省鸡源大肠杆菌中流行的ESBLs基因型与国内其他地区相关报道大体一致,而检测到的bla FOX型AmpC酶基因在国内报道相对较少。To understand the genotype of extend spectrumβ-lactamase(ESBLs)and AmpCβ-lactamase(AmpC)of Escherichia coli isolated from chicken and actuality of antibiotics resistance in Shanxi province,68 isolated strains accorded with biological traits of chicken Escherichia coli were identified from epidemic materials possessed typical clinical symptoms of chicken colibacillosis.These isolated strains were subjected to K-B disc diffusion and double-disc synergy confirmatory methods recommended by CLSI for drug sensitivity test and resistant phenotype;Genotype of ESBLs and AmpC were detected by PCR method.The results showed that all strains presented resistance to 22 antibiotics in different degrees.The resistant spectrum of 66 strains,accounting for 97.06%,reached to above seven kinds of antibiotics;The number of ESBLs-positive,AmpC-positive and double positive strains were 57(83.82%),19(27.94%)and 16(23.53%),respectively;Resistant genotypes of ESBLs-positive strains were bla TEM,bla OXA and bla CTX-M-1/9,and the genotype of AmpC-positive strains was bla FOX.The results of this research indicated that all isolated strains presented resistance to a majority of antibiotics,and the ESBLs-or/and AmpC-producing strains had been prevalent widely.Genotypes of ESBLs prevalent in Shanxi province were in accordance with those in other regions of China,whereas the bla FOX genotype of AmpC detected in our test was relatively less reported in domestic.

关 键 词：鸡 大肠杆菌 ESBLS AMPC 耐药性 基因型 

分 类 号：S852.61[农业科学—基础兽医学]