Quinclorac Resistance in Echinochloa crus-galli from China  

作　　者：PENG Qiong HAN Heping YANG Xia BAI Lianyang YU Qin Stephen BPOWLES 

机构地区：[1]Hunan Agricultural Biotechnology Research Institute,Hunan Academy of Agricultural Sciences,Changsha 410125,China [2]Collaborative Innovation Center for Field Weeds Control,Hunan University of Humanities,Science and Technology,Loudi 417000,China [3]Australian Herbicide Resistance Initiative,School of Agriculture and Environment,University of Western Australia,WA 6009,Australia [4]Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China

出　　处：《Rice science》2019年第5期300-308,共9页水稻科学（英文版）

基　　金：financially supported by the National Natural Science Foundation of China (Grant Nos. 31701803 and 31772182);the Natural Science Foundation of Hunan Province (Grant No. 2017JJ3164) in China and the Australian Grains Research and Development Corporation (GRDC)

摘　　要：Echinochloa crus-galli is a major weed in rice fields in China,and quinclorac has been long used for its control.Over-reliance of quinclorac has resulted in quinclorac resistance in E.crus-galli.Two resistant(R)E.crus-galli populations from Hunan,China were confirmed to be at least 78-fold more resistant to quinclorac than the susceptible(S)population.No difference in foliar uptake of 14C-labelled quinclorac was detected between the R and S plants.However,a higher level of 14C translocation and a lower level of quinclorac metabolism were found in the R plants.Basal and induced expression levels ofβ-cyanoalanine synthase(β-CAS)gene andβ-CAS activity were not significantly different between the R and S plants.However,the induction expression of 1-aminocyclopropane-1-carboxylic acid oxidase(ACO1)gene by quinclorac treatment was evident in the S plants but not in the R plants.Quinclorac resistance in the two resistant E.crus-galli populations was not likely to be related to foliar uptake,translocation or metabolism of quinclorac,nor to cyanide detoxification viaβ-CAS.Thus,target-site based quinclorac signal reception and transduction and regulation of the ethylene synthesis pathway should be the focus for further research.Echinochloa crus-galli is a major weed in rice fields in China, and quinclorac has been long used for its control. Over-reliance of quinclorac has resulted in quinclorac resistance in E. crus-galli. Two resistant(R) E. crus-galli populations from Hunan, China were confirmed to be at least 78-fold more resistant to quinclorac than the susceptible(S) population. No difference in foliar uptake of 14 C-labelled quinclorac was detected between the R and S plants. However, a higher level of 14 C translocation and a lower level of quinclorac metabolism were found in the R plants. Basal and induced expression levels of β-cyanoalanine synthase(β-CAS) gene and β-CAS activity were not significantly different between the R and S plants. However, the induction expression of 1-aminocyclopropane-1-carboxylic acid oxidase(ACO1) gene by quinclorac treatment was evident in the S plants but not in the R plants. Quinclorac resistance in the two resistant E. crus-galli populations was not likely to be related to foliar uptake, translocation or metabolism of quinclorac, nor to cyanide detoxification via β-CAS. Thus, target-site based quinclorac signal reception and transduction and regulation of the ethylene synthesis pathway should be the focus for further research.

关 键 词：ECHINOCHLOA crus-galli QUINCLORAC RESISTANCE QUINCLORAC metabolism β-cyanoalanine SYNTHASE 1-aminocyclopropane-1-carboxylic ACID SYNTHASE 1-aminocyclopropane-1-carboxylic ACID OXIDASE rice 

分 类 号：X59[环境科学与工程—环境工程]