miR-9对人牙周膜干细胞功能的影响  

Effects of miR-9 on the function of human periodontal ligament stem cells

作　　者：郝甍 肖湘水 谢晓莉[2] 唐健霞[1] HAO Meng;XIAO Xiangshui;XIE Xiaoli;TANG Jianxia(Department of Prosthodontics,Xiangya Stomatological Hospital,Central South University,Changsha 410000,China;Department of Conservative Dentistry and Endodontics,Xiangya Stomatological Hospital,Central South University,Changsha 410000,China)

机构地区：[1]中南大学湘雅口腔医院修复科,湖南长沙410000 [2]中南大学湘雅口腔医院牙体牙髓科,湖南长沙410000

出　　处：《口腔生物医学》2019年第3期114-117,共4页Oral Biomedicine

基　　金：国家自然科学青年基金(81500812);中南大学研究生科研创新项目(2018zzts840)

摘　　要：目的:探讨miR-9对人牙周膜干细胞(PDLSCs)增殖、迁移和成骨能力的调控作用。方法:分离和培养人PDLSCs,瞬时转染miR-9 inhibitor片段后,使用Brdu法、细胞划痕实验、茜素红染色和Western blot法分别检测PDLSCs的增殖、迁移和成骨能力的改变。结果:瞬时转染miR-9 inhibitor后,PDLSCs的增殖能力减弱,细胞迁移速度变慢,成骨能力受损(P<0.05)。结论:miR-9可调控PDLSCs的增殖、迁移和成骨能力。Objective:To observe the effects of miR-9 on the proliferation,migration and osteogenic differentiation of human periodontal ligament stem cells(PDLSCs)in vitro.Methods:PDLSCs were isolated and cultured in vitro,and were transfected with miR-9 inhibitor.Proliferation of PDLSCs was evaluated by Brdu assay.Migration of PDLSCs was tested by cell wound scratch assay.Osteogenic differentiation of PDLSCs was determined by Alizarin red staining and Western blot.Results:Suppression of the function of miR-9 could reduce proliferation of PDLSCs,lowered the migration speed of PDLSCs,and harmed the osteogenic differentiation of PDLSCs(P<0.05).Conclusions:miR-9 could regulate the proliferation,cell migration and osteogenic differentiation of PDLSCs.

关 键 词：MIR-9 牙周膜干细胞 增殖 迁移 成骨 

分 类 号：R780.2[医药卫生—口腔医学]