可溶性环氧化物水解酶抑制剂TPPU治疗周围神经痛效果研究  

Study of the effect of soluble epoxide hydrolase inhibitor TPPU in the treatment of peripheral neuralgia

作　　者：张琳[1,2] 张耀扬[1] 白杰 王福 ZHAG Lin;ZHANG Yaoyang;BAI Jie;WANG Fu(School of Stomatology,Dalian Medical University,Dalian 116044,China;Department of Stomatology,Affiliated Hospital of Qingdao University,Qingdao 277000,China)

机构地区：[1]大连医科大学口腔医学院,辽宁大连116044 [2]青岛大学附属医院口腔科,山东青岛277000

出　　处：《口腔生物医学》2019年第3期123-126,共4页Oral Biomedicine

基　　金：国家自然科学基金(81771032)

摘　　要：目的:探究可溶性环氧化物水解酶抑制剂1-(三氟甲氧基苯基)-3-(1-丙酰基哌啶-4-基)脲(TPPU)治疗周围神经痛的效果。方法:选择SD大鼠,通过坐骨神经慢性压迫损伤建立坐骨神经痛模型。术前3 d至术后7 d给予(实验组)或不给予(对照组)TPPU灌胃处理。术后使用von Frey测试SD大鼠痛阈,监测体质量变化,免疫组织化学染色观察神经新生血管数量及坐骨神经脊髓段尼氏小体表达。结果:实验组疼痛阈值和体质量增加明显高于对照组(P<0.05);免疫荧光结果显示实验组局部微循环改善,尼氏小体表达高于对照组(P<0.05)。结论:TPPU可能通过改善微循环促进神经损伤修复,减少神经损伤性疼痛。Objective:To study the effects of soluble epoxide hydrolase inhibitor 1-(trifluoromethoxyphenyl)-3-(1-propionylpiperidin-4-yl)urea(TPPU)on peripheral neuralgia by targeted blockade of soluble epoxide hydrolase.Methods:The chronic neuropathic pain was generated by chronic constriction injury of the sciatic nerve in SD rats.The rats were administered with TPPU(experimental group)or without TPPU(control group)by gastric lavage daily 3 days before surgery until 7 days after surgery.The body weight and pain threshold(von Frey assay)were measured.Immunohistochemical staining was used to observe the number of nerve neovascularization and the expression of Nissl bodies in the sacral part of the spinal cord.Results:Pain threshold and body weight gain in the experimental group were significantly higher than those in the control group(P<0.05).Immunofluorescence results showed that the local microcirculation was improved in the TPPU-treated group,and the expression of Nissl bodies was higher than that in the control group(P<0.05).Conclusions:TPPU targeted blockade of soluble epoxide hydrolasemay promoted nerve damage repair and reduced nerve injury pain by improving the microcirculation.

关 键 词：周围神经痛 环氧化物水解酶抑制剂 1-(三氟甲氧基苯基)-3-(1-丙酰基哌啶-4-基)脲 微循环 

分 类 号：R780.2[医药卫生—口腔医学]