阿福豆苷对结直肠癌小鼠肿瘤抑制作用及其抗血管生成的影响  

Effects of afzelin in treatment of BALA/C mice with colorectal cancer and its influence on anti-angiogenesis

作　　者：邢通潮 祝普利 尹超 马师洋[2] 高鑫原 XING Tongchao;ZHU Puli;YIN Chao;MA Shiyang;GAO Xinyuan(The Fourth People′s Hospital of Shaanxi Province,Xi’an 710000,China)

机构地区：[1]陕西省第四人民医院,西安710000 [2]西安交通大学第二附属医院 [3]西安市中医医院

出　　处：《山东医药》2019年第23期39-43,共5页Shandong Medical Journal

基　　金：陕西省自然科学基金资助项目(2013JM4016)

摘　　要：目的探讨阿福豆苷对结直肠癌BALA/C小鼠肿瘤抑制作用及抗血管生成作用。方法将60只BALA/C小鼠随机分为顺铂组、模型组、高剂量阿福豆苷组、中剂量阿福豆苷组及低剂量阿福豆苷组,每组各12只。接种结直肠癌LOVO细胞进行结直肠癌造模,对高剂量阿福豆苷组、中剂量阿福豆苷组及低剂量阿福豆苷组小鼠分别灌胃阿福豆苷100、50、25 mg/kg;顺铂组给予顺铂15 mg/kg灌胃;模型组给予等量生理盐水灌胃。各组均灌胃1次/d,连续给药30 d。末次灌胃24 h后,将各组小鼠处死,取其肿瘤组织,称取肿瘤组织重量,计算肿瘤抑制率及肿瘤组织体积。采用HE染色法观察各组肿瘤组织病理学情况。采用荧光免疫法检测各组小鼠血管生成相关蛋白血管内皮生长因子(VEGF)、低氧诱导因子1α(HIF-1α)及血管内皮生长因子受体2(VEGFR-2)表达情况。结果与模型组比较,顺铂组、高剂量阿福豆苷组、中剂量阿福豆苷组和低剂量阿福豆苷组小鼠肿瘤组织重量及肿瘤组织体积均降低(P均<0.05)。HE染色结果显示,模型组小鼠肿瘤组织中癌细胞形态及染色情况均正常,癌细胞结构完整,高剂量阿福豆苷组、中剂量阿福豆苷组和低剂量阿福豆苷组小鼠肿瘤组织癌细胞亦出现大面积凋亡。与模型组比较,顺铂组、高剂量阿福豆苷组、中剂量阿福豆苷组和低剂量阿福豆苷组小鼠肿瘤组织VEGFR-2、VEGF及HIF-1α相对表达量均降低(P均<0.05)。结论阿福豆苷对结直肠癌BALA/C小鼠具有较好的肿瘤抑制作用,其主要机制可能为抗血管生成。Objective To observe the effects of afzelin in the treatment of BALA/C mice with colorectal cancer and its influence on the anti-angiogenesis.Methods Sixty BALA/C mice were divided into the control group,cis-platinum group(20 mg/kg),high-dose afzelin group(100 mg/kg),medium-dose afzelin group(50 mg/kg)and low-dose afzelin group(25 mg/kg).Using LOVO colorectal cancer cells for axillary vaccination to build the colorectal cancer models.After the models were built,the afzelin was gavaged for thirty days.At 24 hours after the last gavage,mice in each group were sacrificed and the tumor tissues were taken,and the weight of tumor tissues,tumor inhibition rate and tumor volume were observed.The tumor tissues were divided into 4 parts,the histopathologies were observed by HE staining in one part,while the vascular endothelial growth factor(VEGF),hypoxia-inducible factor 1-α(HIF-1α)and vascular endothelial growth factor receptor(VEGFR-2)were detected by fluorescence immunoassay in other three parts in the five groups.Results Compared with the model group,the tumor volume and weight were significantly lower in the high-dose,medium-dose,and low-dose afzelin groups(both P<0.05).HE staining showed that the morphology and staining of cancer cells in the tumor tissues of the model group were normal,and the structure was intact.The tumor cells in the high-dose,medium-dose,and low-dose afzelin groups had large areas of apoptosis.Compared with the model group,the expression of VEGF,HIF-1αand VEGFR-2 decreased significantly in the high-dose,medium-dose,and low-dose afzelin groups(all P<0.05).Conclusion Afzelin in the treatment of BALA/C mice with colorectal cancer is effective,and has good anti-angiogenesis effects.

关 键 词：阿福豆苷 结直肠癌 BALA/C小鼠 抗血管生成 

分 类 号：R730.23[医药卫生—肿瘤] R735.3[医药卫生—临床医学]