钙蛋白酶小亚基1在乳腺癌中的表达及其临床意义  

Expression of calpain small subunit 1 in breast cancer and its clinical significance

作　　者：朱烨丰[1] 王尊[1] 姜敏 史超颖 王晶[2] Zhu Yefeng;Wang Zun;Jiang Min;Shi Chaoying;Wang Jing(Department of Breast Surgery,Maternal and Child Health Care Hospital of Bao'an,Shenzhen 518133,China;Department of Pathology,Shanghai General Hospital,Shanghai 200080,China)

机构地区：[1]深圳市宝安区妇幼保健院乳腺科,518133 [2]上海市第一人民医院病理科,200080

出　　处：《肿瘤研究与临床》2019年第8期530-534,共5页Cancer Research and Clinic

基　　金：深圳市宝安区科技计划社会公益项目(医疗卫生类)(2015102)。

摘　　要：目的探讨钙蛋白酶小亚基1(Capn4)在乳腺癌组织中的表达及其临床意义.方法收集上海市第一人民医院2015年5月至7月收治的12例乳腺癌患者的肿瘤组织及相应癌旁正常组织存档蜡块标本,运用免疫组织化学法检测其中Capn4的表达情况.收集上海市第一人民医院2009年1月至2011年9月收治的70例乳腺癌患者的肿瘤组织存档蜡块标本并检测其中Capn4的表达情况,探讨其与乳腺癌TNM分期、病理学分级、分子标志物及预后的相关性.结果乳腺癌组织中Capn4的表达均高于相应癌旁正常组织.Capn4的表达水平与乳腺癌患者的TNM分期(P=0.002)、T分期(P=0.004)、N分期(P=0.004)、M分期(P=0.025)、雌激素受体状态(χ^2=4.787,P=0.029)、生存状态(χ^2=5.826,P=0.016)均相关.Kaplan-Meier法显示Capn4高表达患者的中位生存时间(90个月)短于Capn4低表达患者(未达到),差异有统计学意义(χ^2=4.351,P=0.037).结论Capn4在乳腺癌组织中表达上调,其表达差异与乳腺癌TNM分期、病理学分级、雌激素受体状态、生存预后相关,提示其可能成为乳腺癌治疗新的靶点和分子标志物.Objective To investigate the expression of calpain small subunit 1 (Capn4) in breast cancer and its clinical significance. Methods The cancer tissues and the paraffin-embeded specimens of the corresponding adjacent normal tissues from 12 patients with breast cancer between May 2015 and July 2015 in Shanghai General Hospital were collected. The expression of Capn4 was detected by using immunohistochemistry. The paraffin-embeded specimens of cancer tissues from 70 breast cancer patients in Shanghai General Hospital between January 2009 and September 2011 were also collected. And the expression of Capn4 was detected. The correlation between Capn4 expression level and TNM stage, pathological grade, molecular markers as well as the prognosis of breast cancer was also analyzed. Results The expression of Capn4 in breast cancer tissues was higher than that in the corresponding adjacent normal tissues. The expression level of Capn4 was correlated with TNM stage (P = 0.002), T stage (P = 0.004), N stage (P = 0.004), M stage (P = 0.025), estrogen receptor (ER) status (χ 2 = 4.787, P = 0.029) and survival status (χ 2 = 5.826, P = 0.016). Kaplan-Meier survival analysis revealed that the median survival time in the higher expression of Capn4 group 90 months was shorter than that in the lower expression of Capn4 group (not reaching the median survival time), and there were statistical differences (χ 2 = 4.351, P = 0.037). Conclusions The expression of Capn4 in breast cancer tissues is upregulated. The expression difference is correlated with TNM stage, pathological grade, ER status and the prognosis of breast cancer, suggesting that it may become a new target and molecular marker for breast cancer treatment.

关 键 词：乳腺肿瘤 钙激活中性蛋白酶 钙蛋白酶小亚基1 预后 免疫组织化学 

分 类 号：R73[医药卫生—肿瘤]