樟树叶精油抗PM2.5致肺上皮细胞损伤的研究  

Antagonistic effect of camphor leaf essential oil on damage induced by PM2.5 in lung epithelial cells A549

作　　者：熊国红 林海 何建国 李应洪[2] 付湘晋[2] XIONG Guo-hong;LIN Hai;HE Jian-guo;LI Ying-hong;FU Xiang-jin(Yiyang Institute of Commodity Quality Supervision and Inspection,Yiyang,Hunan 413000,China;College of Food Science and Engineering,Central South University of Forestry and Technology,Changsha,Hunan 410004,China)

机构地区：[1]益阳市产商品质量监督检验研究院,湖南益阳413000 [2]中南林业科技大学食品科学与工程学院,湖南长沙410004

出　　处：《食品与机械》2019年第9期169-172,共4页Food and Machinery

基　　金：国家科技支撑计划（编号：2012BAC01B07-2）;国家林业公益行业科研专项（编号：201104044）

摘　　要：以肺细胞A549为模型,研究樟树叶精油对PM2.5所致细胞损伤的拮抗活性。研究结果显示:樟树叶精油1.0 mg/mL以内对A549细胞增殖无影响,0.05~1.00 mg/mL樟树叶精油对PM2.5所致的细胞毒性作用有显著拮抗作用(P<0.05);樟树叶精油可显著降低染毒细胞中丙二醛(MDA)含量,增加超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性和总抗氧化物质(T-AOC)含量(P<0.05),表明樟树叶精油可通过抗氧化应激拮抗PM2.5致细胞毒性;樟树叶精油可显著降低染毒细胞中炎性因子TNF-α、IL-6的含量(P<0.05),表明樟树叶精油可通过抑制炎症反应拮抗PM2.5致细胞毒性。In this paper,the lung cell A549 was used as a model.The protective effect of camphor(C.camphora)leaf essential oil on the A549 exposed to PM2.5 was investigated.Less than 1 mg/mL of essential oil had no effect on A549 cell proliferation,and low concentration had a certain promotion effect.0.05~1.00 mg/mL essential oil had a significant antagonistic effect on cytotoxicity induced by PM2.5(P<0.05).The essential oilsignificantly reduced the content of MDA in the infected cells and increased the activity of SOD and CAT and the content of T-AOC(P<0.05),indicating that the camphor leaf essential oil could antagonize the cytotoxicity of PM2.5 through antioxidant stress.The camphora leaf essential oilsignificantly reduced the content of TNF-αand IL-6 in the infected cells(P<0.05),indicating that the camphor leaf essential oil could antagonize the cytotoxicity of PM2.5 by inhibiting the inflammatory reaction.

关 键 词：樟树叶精油 PM2.5 细胞毒性 抗氧化 炎性因子 

分 类 号：R28[医药卫生—中药学]