抗菌肽NK-lysin生物信息学分析及其抑菌作用研究  

Bioinformatics Analysis of Antibacterial Peptide NK-lysin and Research on Its Antibacterial Activity

作　　者：赖隆永 刘友霖 刘小龙 谭礼宁 吴昌标[2,3] 杨慧 余勋信[2] 杨守深[4] 徐磊[2,5] LAI Long-yong;LIU You-lin;LIU Xiao-long;TAN Li-ning;WU Chang-biao;YANG Hui;YU Xun-xin;YANG Shou-shen;XU Lei(Present(FuZhou)Biotech Company limited,FuZhou,350500,China;Fujian Vocational College of Agriculture,Fuzhou 350303,China;Fujian Vocational College of Bioengineering,Fuzhou 350007,China;College of Life Sciences,Longyan University,Longyan 364012,China;Nanjing Tianbang Bio-industry Company Limited,Nanjing 211102,China)

机构地区：[1]派生特(福州)生物科技有限公司,福建福州350500 [2]福建农业职业技术学院,福建福州350303 [3]福建生物工程职业技术学院,福建福州350007 [4]龙岩学院生命科学学院,福建龙岩364012 [5]南京天邦生物科技有限公司,江苏南京211102

出　　处：《江西农业学报》2019年第9期95-101,共7页Acta Agriculturae Jiangxi

基　　金：福建省科技计划项目(2019N0025、2017N0009、2016S0002);2018年福建省中青年教师教育科研项目(职业院校专项)(JZ180531);2018年福建省禽病防治重点实验室开放基金课题(KF201902);福建农业职业技术学院福建省动物保健与食品安全应用技术协同创新中心项目[闽教科(2017)49号];福建省畜禽疫病防控技术重大研发平台项目(2014N2003);2019年度江苏省博士后科研资助计划[苏人社发(2019)104号];2019年度福州市科技计划项目(2019-G-34)

摘　　要：为分析猪源抗菌肽NK-lysin的理化性质和蛋白结构,并研究猪源抗菌肽在小鼠体内的抑菌效果,以猪小肠组织cDNA为模板进行PCR扩增和测序,通过生物信息学相关软件和工具对其测序结果进行分析。试验选用60只小鼠,随机分为4组(预防组、细菌模型组、治疗组和对照组),设置不同的大肠杆菌和猪源抗菌肽给药方案。结果表明,猪源抗菌肽NK-lysin为稳定的,半衰期在大肠杆菌中(体内)长,与白细胞介素-2(Interleukin-2,IL-2)和颗粒性蛋白B(Granzyme B,GZMB)具有相互作用;预防组和治疗组小鼠存活率分别为80.00%和86.67%。综上所述,猪源抗菌肽在小鼠体内安全且具有一定的抑菌作用。Physicochemical properties and protein structure of porcine antibacterial peptide NK-lysin were analyzed,and the antibacterial effect of porcine antibacterial peptides in mice was studied in the paper.The PCR amplification and sequencing were performed using porcine small intestine tissue cDNA as a template.Sequencing results were analyzed by bioinformatics related software and tools.Sixty mice were randomly divided into 4 groups according to test requirements,and 15 mice in each group were divided into a prevention group,a bacterial model group and a control group.Different treatments with porcine antibacterial peptide and E.coli were conducted.Results showed that porcine antibacterial peptide NK-lysin was stable,and its half-life was long in E.coli(in vivo),interacting with Interleukin-2(IL-2)and Granzyme B(GZMB).The survival rates of the prevention group and the treatment group were 80.00%and 86.67%,respectively.In conclusion,porcine antibacterial peptide was safe and had certain antibacterial activity in mice.

关 键 词：猪源抗菌肽 抑菌 生物信息学 大肠杆菌 预防 

分 类 号：S852.6[农业科学—基础兽医学]