miR-494在胃癌组织中的表达及其对人胃癌SGC-7901细胞增殖和迁移的影响  

Expression of miR-494 in gastric cancer tissues and its influences on proliferation and migration of human gastric cancer SGC-7901 cells

作　　者：李炜[1] 王娟毅[1] 姚忠强[1] 贺启华[1] 吴光辉 LI Wei;WANG Juan-yi;YAO Zhong-qiang;HE Qi-hua;WU Guang-hui(Department of Oncology,3201 Hospital Affiliated to Xi’an Jiaotong University,Hanzhong 723000,Shaanxi,China)

机构地区：[1]西安交通大学附属汉中三二〇一医院肿瘤内科

出　　处：《现代消化及介入诊疗》2019年第9期952-956,共5页Modern Interventional Diagnosis and Treatment in Gastroenterology

基　　金：三二〇一医院科研基金项目(3201yk201530)

摘　　要：目的探究miR-494在胃癌组织中的表达及其对人胃癌SGC-7901细胞增殖和迁移的影响机制。方法收集34例胃癌病人肿瘤标本和癌旁组织,使用RT-PCR检测miR-494表达情况;将胃癌SGC-7901细胞分为:空白对照组、空白质粒导入组和目的基因组。空白质粒导入组使用空白质粒转染胃癌SGC-7901细胞,目的基因组使用miR-494转染胃癌SGC-7901细胞;分别检测各组miR-494的表达水平;使用CCK-8检测各组胃癌SGC-7901细胞的增殖情况;使用Transwell检测各组胃癌SGC-7901细胞侵袭性及迁移性;使用蛋白质印记检测各组增殖、侵袭和迁移相关蛋白表达水平。结果相比癌旁正常组织,胃癌组织中miR-494表达水平显著降低(P<0.05);空白对照组与空白质粒导入组各项指标均无显著差异(P>0.05);相比空白质粒导入组,目的基因组miR-494表达水平、单位面积细胞侵袭数目和Ki67、VEGF、E-cadherin蛋白表达水平显著降低(P<0.05),细胞抑制率和Vimentin、N-cadherin蛋白表达水平显著升高(P<0.05)。结论miR-494的表达可以有效降低胃癌SGC-7901细胞相关增殖蛋白的表达,抑制其增殖,并通过调节上皮间质转化抑制胃癌SGC-7901细胞的侵袭及迁移能力。Objective To explore the expression of miR-494 in gastric cancer tissues and influence mechanism on proliferation and migration of human gastric cancer SGC-7901 cells.Methods Tumor specimens and adjacent tissues from 34 patients with gastric cancer were collected and expression of microRNA-494 was detected by RT-PCR.The gastric cancer SGC-7901 cells were divided into blank control group,blank plasmid introduction group and target gene group.In blank plasmid introduction group,blank plasmid was transfected into gastric cancer SGC-7901 cells.In target gene group,miR-494 was transfected into gastric cancer SGC-7901 cells.The expression level of miR-494 in each group was detected.The proliferation of gastric cancer SGC-7901 cells in each group was detected by CCK-8.The invasion and migration of gastric cancer SGC-7901 cells in each group were detected by Transwell.The expression levels of proliferation,invasion and migration related protein in each group were detected by Western blotting.Results Compared with that in adjacent normal tissues,expression level of miR-494 was significantly decreased in gastric cancer tissues(P<0.05).There was no significant difference in all indexes between blank control group and blank plasmid introduction group(P>0.05).Compared with blank plasmid introduction group,miR-494 expression level,number of cell invasion per unit area,expression levels of Ki67,VEGF and E-cadherin protein were significantly decreased in target gene group(P<0.05),while cell inhibition rate,expression levels of Vimentin and N-cadherin protein were significantly increased(P<0.05).Conclusion The expression of miR-494 can effectively reduce expression of gastric cancer SGC-7901 cell-associated proliferation protein,inhibit the proliferation,and inhibit invasion,and migration ability of gastric cancer SGC-7901 cells by regulating epithelial-mesenchymal transition.

关 键 词：胃癌SGC-7901 miR-494 细胞增殖 细胞迁移 

分 类 号：R573[医药卫生—消化系统]