中国荷斯坦牛HH4遗传缺陷单倍型分子筛查  

Carrier Screening for Genetic Defect HH4 in Chinese Holstein cattle

作　　者：李艳华[1] 吕小青[1] 刘林[1] 麻柱[1] LI Yan-hua;LV Xiao-qing;LIU Lin;MA Zhu(Beijing Dairy Cattle Center,Beijing 100192;Key Laboratory of Dairy Cattle Genetic,Breeding and Reproduction,Ministry of Agriculture and Rural Affairs,Beijing 100192;Beijing Key Laboratory of Dairy Cattle Genetic,Breeding and Reproduction,Beijing 100192)

机构地区：[1]北京奶牛中心/农业农村部奶牛遗传育种与繁殖重点实验室/奶牛遗传育种与繁殖北京市重点实验室

出　　处：《中国奶牛》2019年第8期29-31,共3页China Dairy Cattle

基　　金：北京市科技计划课题(D171100002417001);国家863课题(2013AA102504)

摘　　要：HH4(Holstein Haplotype 4)是在荷斯坦牛群中发现的一种隐性遗传缺陷单倍型,由1号染色体上GART基因g.1277227A>C单碱基错义突变引起,隐性基因纯合时引起胚胎早期流产,严重威胁着奶牛养殖者的经济效益。本研究利用飞行时间质谱技术对北京地区332头荷斯坦公牛样品和1 151头母牛样品进行了检测分析,结果表明,在所检测的荷斯坦牛群体中,未发现HH4携带者个体,即均为正常个体。提示HH4单倍型对我国荷斯坦牛群影响较小,在奶牛场的选种选配中可以不予考虑,但在遗传物质引进的时候应重点关注,避免引入有害基因。HH4 is an autosomal recessively inherited disorder recently identified in Holstein cattle.The molecular mechanism of HH4 has been identified as a missense mutation g.1277227A>C in GART gene on chromosome 1(BTA1),which leads to loss of gene function.The embryo with homozygous defective alleles will die during early pregnancy,which seriously threatens the economic benefits of dairy farmers.In this study,DNA samples from Holstein bulls(n=332)and cows(n=1151)in Beijing district were detected by MALDI-TOF-MS.As a result,no carrier individuals were identified,that is to say,all individuals are normal.It is suggested that HH4 has little influence on Chinese Holstein cattle and it can be ignored in the breeding and selection of dairy farms.However,we should pay attention to the introduction of genetic material to avoid an influx of harmful genes.

关 键 词：荷斯坦牛 HH4 基因筛查 

分 类 号：S823.3[农业科学—畜牧学]