丙酮酸乙酯联合顺铂作用裸鼠肝移植瘤的研究  

作　　者：李超男 许钰云 黄任锦 吴振兴[1] 

机构地区：[1]杭州医学院

出　　处：《科学技术创新》2019年第27期18-21,共4页Scientific and Technological Innovation

基　　金：浙江省省教育厅科研一般项目（Y201636957）;杭州医学院校级博士启动金（2016B04）;浙江省大学生科技创新活动计划(新苗人才计划)(2019R425004)

摘　　要：目的:研究丙酮酸乙酯(EP)与顺铂(CDDP)联合处理裸鼠对裸鼠肝癌HepG2移植瘤的作用效果,及对个体的副作用;方法:为裸鼠接种p-Gluc荧光标记的HepG2细胞,分为PBS处理组、CDDP(9.6mM)与EP(60μg)单独注射组、CDDP与EP联合注射组;对各组裸鼠的个体体重、移植瘤体积进行观察测量;结果:药物联合组的肿瘤体积显著小于对照组(*P=0.0146),而CDDP处理组无显著变化。药物联合组与CDDP组中肿瘤抑制率分别为36.8%和62.9%;药物联合组体重平均为21g,CDDP组为19g,EP组与对照组约为25g;结论:EP是CDDP增效剂,药物联合处理有望为肝癌治疗方案提出新的思路,并可能缓解化疗药物单独使用带来的副作用。Objective:To study the effect of ethyl pyruvate(EP)combined with cisplatin(CDDP)on HepG2 transplanted tumor in nude mice and the side effects on individuals;Method:Inoculating p-Gluc fluorescently labeled HepG2 cells into nude mice,then divided into PBS-treated group,CDDP(9.6 mM)and EP(60μg)Separate injection group,and CDDP-EP combined injection group;O bserving and measuring individual body weight and transplanted tumor volume of each group;Results:The tumor volume of CDDP-EP therapy group was significantly smaller than that of the control group(*P=0.0146),while there was no significant change in the CDDP therapy group.The tumor inhibition rates in CDDP-EP therapy group and the CDDP group were 36.8%and 62.9%,respectively;the average body weight of CDDP-EP therapy group was 21g,the CDDP group was 19g,while the EP group and the control group were nearly 25g;Conclusion:EP is a CDDP synergist,the drug combination therapy is expected to provide new ideas for the treatment of liver cancer,and may alleviate the side effects caused by the separate use of chemotherapy drugs.

关 键 词：顺铂 丙酮酸乙酯 裸鼠 肝癌移植瘤 

分 类 号：R73-3[医药卫生—肿瘤]