酸性微环境对大肠癌细胞自噬及侵袭能力的影响  

Effects of Acidic Microenvironment on the Invasiveness and Autophagy in Colorectal Cancer Cells

作　　者：占义军[1] 许东强[1] 李明[2] 谭诗云[2] Zhan Yijun;Xu Dongqiang;Li Ming(Xiangyang Central Hospital,Affiliated Hospital of Hubei College of Arts and Science,Hubei 441021,China)

机构地区：[1]襄阳市中心医院、湖北文理学院附属医院,441021 [2]武汉大学人民医院,430060

出　　处：《医学研究杂志》2019年第9期118-121,共4页Journal of Medical Research

基　　金：中央高校基本科研业务费专项资金资助项目(2042017kf0092)

摘　　要：目的本研究探讨酸性环境对大肠癌LoVo细胞侵袭能力及自噬水平的影响。方法选择大肠癌LoVo细胞作为研究对象,用调节后pH值6.8或pH值7.4的全培养基体外培养大肠癌LoVo细胞,Transwell小室法检测细胞侵袭能力的改变;免疫荧光检测细胞膜E-cadherin表达变化情况;蛋白质印迹检测细胞EMT标志物抗体(E-cadherin、Vimentin、Fibronectin)、自噬相关基因Atg5、LC3-Ⅱ和Beclin1蛋白、MMP-9表达变化情况。结果Transwell侵袭实验结果显示,酸性环境下穿过Transwell小室基膜的大肠癌细胞数为491.4±19.5个,多于中性环境(pH值7.4)的353.8±24.6个,差异有统计学意义(t=7.59,P=0.002);酸性微环境可使LoVo细胞EMT标志物蛋白E-cadherin表达降低,且Vimentin、Fibronectin蛋白表达上调,同时MMP-9蛋白表达上调;且自噬相关基因Atg5、LC3-Ⅱ和Beclin1蛋白表达上调。结论酸性环境可使细胞侵袭能力增加,其机制可能通过诱导LoVo细胞自噬和EMT。Objective Explore the effects of acidic microenvironment on the metastasis and autophagy in colorectal cancer cell line LoVo cells.Methods The colorectal cancer cell line LoVo cells were cultured in vitro.And LoVo cells were treated in complete medium,adjusted pH to 6.8 or 7.4.The invasion of LoVo cells was tested by transwell assay.The expression of E-cadherin was analyzed by immunofluorescence staining.Western blot were used for E-cadherin,Vimentin,Fibronectin,MMP-9,Atg5,LC3-Ⅱand Beclin1protein analysis.Results Transwell experiments revealed that the number of LoVo cells under acidic microenvironment passing through the basement membrane was greater than that of the control group(P<0.05),suggesting that acidic microenvironment could enhance the capacity of cell invasion.Acidic microenvironment could promoted the expressions of Vimentin,Fibronectin,MMP-9,Atg5,LC3-Ⅱand Beclin1 protein,and suppressed the expression of E-cadherin.Conclusion Acidic microenvironment could enhance the capacity of cell invasion,which may be associated with promotion of autophagy and epithelial-to-mesenchymal transition in colorectal cancer cell line LoVo cells.

关 键 词：酸性微环境 大肠癌 自噬 上皮间质转化 侵袭 

分 类 号：R73[医药卫生—肿瘤]