Protective effects of organic extracts of Alpinia oxyphylla against hydrogen peroxide-induced cvtotoxicitv in PC12 cells  

作　　者：Li-Hong Duan Meng Li Chun-Bao Wang Qing-Mei Wang Quan-Quan Liu Wan-Feng Shang Ya-Jin Shen Zhuo-Hua Lin Tong-Yang Sun Zheng-Zhi Wu Ying-Hong Li Yu-Long Wang Xun Luo 

机构地区：[1]Shenzhen Institute of Geriatrics,Shenzhen,Guangdong Province,China [2]Department of Rehabilitation,the Second People’s Hospital of Shenzhen,Shenzhen,Guangdong Province,China [3]Stroke Biological Recovery Laboratory,Spaulding Rehabilitation Hospital,Harvard Medical School,Charlestown,MA,USA [4]Department of Mechanical and Biomedical Engineering,City University of Hong Kong,Hong Kong Special Administrative Region,China [5]Department of Rehabilitation,the First Affiliated Hospital of Shenzhen University,Shenzhen,Guangdong Province,China [6]School of Mechanical and Automotive Engineering,South China University of Technology,Guangzhou,Guangdong Province,China [7]School of Mechanical Engineering,Guangxi University of Science and Technology,Liuzhou,Guangxi Zhuang Autonomous Region,China [8]Mingkai Smart Medical Robot Co.,Ltd.,Shenzhen,Guangdong Province,China [9]Kerry Rehabilitation Medicine Research Institute,Shenzhen,Guangdong Province,China

出　　处：《Neural Regeneration Research》2020年第4期682-689,共8页中国神经再生研究（英文版）

基　　金：financially supported by the National Natural Science Foundation of China,No.81574038(to ZZW);the Natural Science Foundation of Guangdong Province of China,No.2017A030313842(to LHD);the Science and Technology Foundation of Guangdong Province of China,No.2017A050506007(to YHL);the Technology Research Foundation of Basic Research Project of Shenzhen City of China,No.JCYJ20170412161254416(to ZZW)

摘　　要：Alpinia oxyphylla,a traditional herb,is widely used for its neuroprotective,antioxidant and memory-improving effects.However,the neuroprotective mechanisms of action of its active ingredients are unclear.In this study,we investigated the neuroprotective effects of various organic extracts of Alpinia oxyphylla on PC12 cells exposed to hydrogen peroxide-induced oxidative injury in vitro.Alpinia oxyphylla was extracted three times with 95%ethanol(representing extracts 1–3).The third 95%ethanol extract was dried and resuspended in water,and then extracted successively with petroleum ether,ethyl acetate and n-butanol(representing extracts 4–6).The cell counting kit-8 assay and microscopy were used to evaluate cell viability and observe the morphology of PC12 cells.The protective effect of the three ethanol extracts(at tested concentrations of 50,100 and 200μg/mL)against cytotoxicity to PC12 cells increased in a concentration-dependent manner.The ethyl acetate,petroleum ether and n-butanol extracts(each tested at 100,150 and 200μg/mL)had neuroprotective effects as well.The optimum effective concentration ranged from 50–200μg/mL,and the protective effect of the ethyl acetate extract was comparatively robust.These results demonstrate that organic extracts of Alpinia oxyphylla protect PC12 cells against apoptosis induced by hydrogen peroxide.Our findings should help identify the bioactive neuroprotective components in Alpinia oxyphylla.Alpinia oxyphylla,a traditional herb,is widely used for its neuroprotective,antioxidant and memory-improving effects.However,the neuroprotective mechanisms of action of its active ingredients are unclear.In this study,we investigated the neuroprotective effects of various organic extracts of Alpinia oxyphylla on PC12 cells exposed to hydrogen peroxide-induced oxidative injury in vitro.Alpinia oxyphylla was extracted three times with 95%ethanol(representing extracts 1-3).The third 95%ethanol extract was dried and resuspended in water,and then extracted successively with petroleum ether,ethyl acetate and n-butanol(representing extracts 4-6).The cell counting kit-8 assay and microscopy were used to evaluate cell viability and observe the morphology of PC12 cells.The protective effect of the three ethanol extracts(at tested concentrations of 50,100 and 200μg/mL)against cytotoxicity to PC12 cells increased in a concentration-dependent manner.The ethyl acetate,petroleum ether and n-butanol extracts(each tested at 100,150 and 200μg/mL)had neuroprotective effects as well.The optimum effective concentration ranged from 50-200μg/mL,and the protective effect of the ethyl acetate extract was comparatively robust.These results demonstrate that organic extracts of Alpinia oxyphylla protect PC12 cells against apoptosis induced by hydrogen peroxide.Our findings should help identify the bioactive neuroprotective components in Alpinia oxyphylla.

关 键 词：active INGREDIENTS ALPINIA oxyphylla apoptosis ethanol crude extract fraction hydrogen PEROXIDE nerve regeneration NEUROPROTECTIVE agent NEUROPROTECTIVE effects PC12 cells traditional HERB 

分 类 号：R452[医药卫生—治疗学] R363[医药卫生—临床医学]