Small-world networks of neuroblastoma cells cultured in three-dimensional polymeric scaffolds featuring multi-scale roughness  

作　　者：Valentina Onesto Angelo Accardo Christophe Vieu Francesco Gentile 

机构地区：[1]Center for Advanced Biomaterials for Healthcare,Italian Institute of Technology,Naples,Italy [2]Laboratoire d’Analyse et d’Architecture des Systemes(LAAS),Centre National de la Recherche Scientifique,Universite de Toulouse,CNRS,Toulouse,France [3]Institut National des Sciences Appliquees–INSA,Toulouse,France [4]Department of Electric Engineering and Information Technology,University Federico II,Naples,Italy [5]Department of Precision and Microsystems Engineering,Delft University of Technology,Mekelweg 2,2628 CD Delft,The Netherlands

出　　处：《Neural Regeneration Research》2020年第4期759-768,共10页中国神经再生研究（英文版）

摘　　要：Understanding the mechanisms underlying cell-surface interaction is of fundamental importance for the rational design of scaffolds aiming at tissue engineering,tissue repair and neural regeneration applications.Here,we examined patterns of neuroblastoma cells cultured in three-dimensional polymeric scaffolds obtained by two-photon lithography.Because of the intrinsic resolution of the technique,the micrometric cylinders composing the scaffold have a lateral step size of^200 nm,a surface roughness of around 20 nm,and large values of fractal dimension approaching 2.7.We found that cells in the scaffold assemble into separate groups with many elements per group.After cell wiring,we found that resulting networks exhibit high clustering,small path lengths,and small-world characteristics.These values of the topological characteristics of the network can potentially enhance the quality,quantity and density of information transported in the network compared to equivalent random graphs of the same size.This is one of the first direct observations of cells developing into 3D small-world networks in an artificial matrix.Understanding the mechanisms underlying cell-surface interaction is of fundamental importance for the rational design of scaffolds aiming at tissue engineering,tissue repair and neural regeneration applications.Here,we examined patterns of neuroblastoma cells cultured in three-dimensional polymeric scaffolds obtained by two-photon lithography.Because of the intrinsic resolution of the technique,the micrometric cylinders composing the scaffold have a lateral step size of～200 nm,a surface roughness of around 20 nm,and large values of fractal dimension approaching 2.7.We found that cells in the scaffold assemble into separate groups with many elements per group.After cell wiring,we found that resulting networks exhibit high clustering,small path lengths,and small-world characteristics.These values of the topological characteristics of the network can potentially enhance the quality,quantity and density of information transported in the network compared to equivalent random graphs of the same size.This is one of the first direct observatioins of cells developing into 3D small-world networks in an artificial matrix.

关 键 词：3D NETWORKS BIOMATERIALS nano-topography network topology neuro-regeneration SMALL-WORLD NETWORKS tissue engineering TWO-PHOTON lithography 

分 类 号：R459.9[医药卫生—治疗学] R319[医药卫生—临床医学]