青光安缓释剂对抗青光眼术后滤过道瘢痕化TGF-β/Smad信号传导通路相关蛋白的影响  

Effects of Qingguangan slow-release agent on the related protein of TGF-β/Smad signal transduction pathway in the fltering scarring after glaucoma filtering surgery

作　　者：欧阳云[1,2] 彭俊 黄学思[1] 李苑碧[1] 陈晓柳 吴权龙 李文娟[1] 谭涵宇[1] 彭清华[1] OUYANG Yun;PENG Jun;HUANG Xue-si;LI Yuan-bi;CHEN Xiao-liu;WU Quan-long;LI Wen-juan;TAN Han-yu;PENG Qing-hua(Hunan University of Chinese Medicine,Changsha 41020B,China;Yancheng Hospital of TCM,Yancheng 224001,China)

机构地区：[1]湖南中医药大学,长沙410208 [2]盐城市中医院,盐城224001

出　　处：《中华中医药杂志》2019年第9期3986-3989,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81273807,No.81403437);湖南省高层次卫生人才“225”工程培养项目资助;教育部博士学科点基金资助项目(No.20124323110006);湖南省自然科学基金重点资助项目(No.11JJ2050);湖南省教育厅科研基金重点资助项目(No.06A052,No.10A094);湖南省研究生创新基金重点资助项目(No.CX2013A013);中医药防治眼耳鼻喉疾病湖南省重点实验室资助项目(No.2017TP1018);国家中医药管理局中医眼科学重点学科建设项目;湖南省中医五官科学重点学科建设项目;湖南中医药大学中医学一级学科资助~~

摘　　要：目的:观察青光安缓释剂对抗青光眼术后滤过道瘢痕化过程中TGF-β/Smad信号传导通路相关蛋白的影响,探讨其作用机制。方法:将48只实验用兔分为空白组、模型组、MMC组、青光安组分1~4组和安慰剂组。除空白组外,其余各组行小梁切除+虹膜根部切除术,并进行相对应的处理。术后眼部常规检查,用药4周后进行术眼HE染色和相关蛋白检测。结果:术后MMC组和青光安组分2组眼压低于其他各组(P<0.05);两组滤过道轮廓清晰,纤维细胞及炎症细胞较其他各组少,且两组CollagenⅠ蛋白量明显低于其他各组(P<0.01);而MMC组Smad3、Smad4的蛋白量明显低于其他各组(P<0.01);结论:青光安组分2能使TGF-β/Smad通路的信号传导减弱,抑制滤过道瘢痕形成,安全性优于MMC。Objective: To observe the effect of Qingguangan slow-release agent on the related protein of TGF-β/Smad signal transduction pathway in the filtering scarring after glaucoma filtering surgery. To explore its mechanisms of Qingguangan slow release agent. Methods: Forty-eight rabbits were randomly divided into control group, model group, MMC group, Qingguanganan group component 1 to 4 group and the placebo group. In addition to the control group, trabeculectomy and irid root resection were performed in the other groups, and corresponding treatment was carried out. Taking routine examination of the eyes after operation, HE staining and related protein detection were performed 4 weeks later. Results: IOP in MMC group and Qingguangan component 2 group was lower than that of other groups(P<0.05). The outline of the filter passage in both groups was clear, and the number of fibroblasts and inflammatory cells in the two groups was less than that in the other groups. The protein content of Collagen Ⅰ in the two groups was significantly lower than that in the other groups(P<0.01). The protein content of Smad3 and Smad4 in MMC group was significantly lower than that in other groups(P<0.01). Conclusion: Qingguangan component 2 can weaken the signal transduction of TGF-β/Smad pathway and inhibit the formation of filtering scar, which is safer than MMC.

关 键 词：青光安缓释剂 青光眼 滤过道瘢痕化 TGF-β/Smad信号传导通路 

分 类 号：R73[医药卫生—肿瘤]