OsAAP6基因在不同氮肥条件下的表达分析及其对水稻籽粒营养品质的影响  

Expression Analysis of OsAAP6 Gene under Different Nitrogen Conditions and Its Effects on Grain Nutritional Quality in Rice

作　　者：彭波 何璐璐 田夏雨 辛晴晴 孙艳芳 宋晓华 孙玲 刘毓琛 庞瑞华 周棋赢 汪全秀 周伟 宋世枝 PENG Bo;HE Lu-Iu;TIAN Xia-yu;XIN Qing-qing;SUN Yan-fang;SONG Xiao-hua;SUN Ling;LIU Yu-chen;PANG Rui-hua;ZHOU Qi-ying;WANG Quan-xiu;ZHOU Wei;SONG Shi-zhi(College of Life Sciences and Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains,Xinyang Normal University,Henan Xinyang 464000,China;Xinyang Academy of Agricultural Sciences,Henan Xinyang 464000,China;College of International Education,Xinyang Normal University,Henan Xinyang 464000,China)

机构地区：[1]信阳师范学院生命科学学院/大别山农业生物资源保护与利用研究院,河南信阳464000 [2]河南省信阳市农业科学院,河南信阳464000 [3]信阳师范学院国际教育学院,河南信阳464000

出　　处：《西南农业学报》2019年第9期1973-1979,共7页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学基金(U1604110,31600992,31801332,U1404319);河南省重点科技攻关项目(182102110442,192102110119);河南省高等学校重点科研项目(19A180030,15A180059);河南省重大科技专项(121100110200);河南省科技惠民项目(162207310009);信阳师范学院研究生科研创新基金项目(2018KYJJ47);信阳师范学院“南湖学者奖励计划”青年项目(2016054);信阳师范学院大学生科研基金项目(2018-DXS-066);大别山农业生物研究院开放课题(2016020)

摘　　要：【目的】调查不同氮肥水平处理对水稻OsAAP6基因表达的影响,并进一步探究OsA4P6基因在不同氮肥条件下对其水稻籽粒中蛋白质(包括谷蛋白、醇溶蛋白、球蛋白和清蛋白)含量和氨基酸含量等营养品质的影响作用,为今后利用OsA4P6基因提高稻米营养品质提供参考。【方法】设置5个氮肥浓度梯度,在水稻OsA4P6遗传材料中分别检测OsA4P6基因的表达水平,并利用近红外谷物分析仪和考马斯亮蓝G-25O法检测分析其成熟种子中蛋白质含量和氨基酸含量等稻米营养品质性状。【结果】随着田间氮肥施加量的增加,OsA4P6基因的表达水平明显提高,并能显著促进水稻籽粒中谷蛋白的积累,进而增加水稻籽粒中总蛋白质的含量;在氮肥浓度较高的条件下基因有助于提高水稻籽粒中总必需氨基酸的含量;随着氮肥施加量的增加,OsA4P6基因能够显著提高水稻籽粒中总氨基酸的含量。【结论】OsA4P6基因随着田间氮肥浓度的增加,其表达水平不断升高,并能促进水稻籽粒中蛋白质含量和氨基酸含量等稻米营养品质的提高,这为今后利用OsA4P6基因提高稻米的营养品质提供了重要参考。【Objective】This paper aimed to investigate the effects of different nitrogen levels on the expression of 0sAAP6 gene in rice and further explore the effects of 0sAAP6 gene on the contents of protein(including glutenin,prolamin,globulin and albumin)and amino acids in rice grains under different nitrogen fertilizer conditions so as to utilize 0sAAP6 gene for improving the nutritional quality of rice in the future.【Method】Five nitrogen concentration gradients were set up to detect the expression level of gene from the genetic materials in rice,and the protein contents and amino acid contents in the mature seeds by near infrared grain analyzer and Coomassie Brilliant Blue G-250 method were analyzed.【Result】With the increase of nitrogen fertilizer concentration in the field,the expression level of 0sAAP6 gene in-creased significantly,which could promote the accumulation of glutenin in rice grain and then increased the total content of grain protein in rice.Simultaneously,with the increase of nitrogenous fertilizer application,0sAAP6 gene could significantly increase the total content of amino acids in rice grain.【Conclusion】gene expression level increased with the increase of nitrogen fertilizer concentration in the field,and could promote the nutritional quality in rice,such as the protein content and amino acids content in the grain,which provided an important reference for the future utilize of 0sAAP6 gene to improve the nutritional quality of rice grain.

关 键 词：氮肥处理 水稻 0sA4P6基因 蛋白质含量 氨基酸含量 

分 类 号：S511[农业科学—作物学]