Na2SeO3对药食用真菌蛹虫草子实体生长及功能成分腺苷、虫草素的影响  

Effect of Sodium Selenite （Na2SeO3） on Fruiting Body Growth, Adenosine and Cordycepin of Functional Components in Medical Edible Fungus of Cordyceps militaris

作　　者：马麦艳 张相锋 马正海[1] 焦子伟 MA Mai-yan;ZHANG Xiang-feng;MA Zheng-hai;JIAO Zi-wei(College of Life Science and Technology,Xinjiang University,Urumqi 830046,China;College of School of Biological and Geographic Sciences,Yili Normal University,Yining Xinjiang 835000,China)

机构地区：[1]新疆大学生命科学学院,乌鲁木齐830046 [2]伊犁师范大学生物与地理科学学院,新疆伊宁835000

出　　处：《新疆农业科学》2019年第8期1510-1517,共8页Xinjiang Agricultural Sciences

基　　金：自治区科技支疆项目(2017E0239);微生物资源保护与开发利用重点实验室开放项目(YLUKLM201606)~~

摘　　要：【目的】研究分析Na 2 SeO 3对药食用真菌蛹虫草子实体生长及功能成分腺苷、虫草素的影响,大面积人工栽培富硒蛹虫草提供理论依据和技术支持。【方法】以新疆本地蛹虫草菌种作为富硒载体,采用瓶栽法,系统分析不同浓度亚硒酸钠处理对蛹虫草菌丝、子座、子实体生长,产量、生物转化率、总硒及其功能成分腺苷、虫草素含量的影响。【结果】处理1(硒浓度20 mg/L)和处理2(硒浓度40 mg/L)与对照相比其蛹虫草的菌丝体生长、子座生长、子实体出草长度、鲜重、干重、生物转化率等无影响,其子实体中总硒含量最高,功能成分腺苷、虫草素含量明显增加;从处理3(硒浓度60 mg/L)至处理7(硒浓度200 mg/L)与对照相比,其蛹虫草的菌丝体生长、子座生长受到抑制,其子实体出草长度、鲜重、干重、生物转化率等呈显著性差异(P<0.05),呈降低趋势,并随着硒浓度增加,抑制越明显;其子实体中总硒含量逐渐降低,功能成分腺苷、虫草素含量逐渐下降。【结论】以亚硒酸钠作为外源硒,硒浓度20~40 mg/L效果最好,可作为进行蛹虫草富硒栽培较为理想的浓度。【Objective】Selenium,as one of the essential trace elements in human body,is dispensable for organism's metabolism.Crodyceps militaris,resembling to Cordyceps sinensis,is an edible and medical fungus which has good selenium enrichment ability.In view of some differences in the biological characteristics of Crodyceps militaris in different regions,it is of great significance both in theory and in application for the further research on the development of natural selenium-rich Crodyceps militaris.【Methods】In this project,local species of Crodyceps militaris in Xinjiang was used as se-carrier,the effects of different concentrations of sodium selenite treatment on the growth,yield,biological conversion rate,total selenium and its functional components adenosine and the content of Crodyceps militaris were systematically analyzed by using the flask planting method.【Results】Treatment 1(with a selenium concentration of 20 mg/L)and Treatment 2(with a selenium concentration of 40 mg/L)had no effects on the growth of mycelium,stroma,fruiting body length,fresh weight,dry weight and biological transformation rate,compared with control group,while the total selenium content,functional component adenosine and cordycepin content was significantly increased;From Treatment 3(60 mg/L selenium)to Treatment 7(200 mg/L selenium),the growth of fruiting body and stroma was suppressed,and the fruiting body length,fresh and dry weight,biological transformation rate were significantly decreased compared with control group(P<0.05),and with the increase of selenium concentration,the suppression increased as well;The total selenium content in fruiting body also declined and adenosine and cordycepin content decreased gradually.【Conclusion】Sodium selenite could be used as exogenous selenium for enriched selenium Crodyceps militaris cultivation,and the pure selenium concentration of 20-40 mg/L displayed the best performance,which could provide theoretical basis and technical support for large-scale cultivation of selenium enriched

关 键 词：亚硒酸钠 蛹虫草 子实体 富硒 虫草素 腺苷 

分 类 号：S188[农业科学—农业基础科学]