叶绿醇对铅污染土壤酶活性及土壤铅有效态影响  

Effects of Phytol on Soil Enzyme Activity and Lead Availability in Lead-contaminated Soil

作　　者：于培鑫 潘芳慧 王友保[1,2] 李晶[1,3] YU Peixin;PAN Fanghui;WANG Youbao;LI Jing(College of Life Sciences,Anhui Normal University,Wuhu,Anhui 241000;Provincial Key Laboratory of Biotic Environment and Ecological Safety in Anhui,Wuhu,Anhui 241000;College of Life Sciences,Bengbu Medical College,Bengbu,Anhui 233000)

机构地区：[1]安徽师范大学生命科学学院,安徽芜湖241000 [2]安徽师范大学生物环境与生态安全安徽省高校省级重点实验室,安徽芜湖241000 [3]蚌埠医学院生命科学学院,安徽蚌埠233000

出　　处：《水土保持学报》2019年第5期358-363,共6页Journal of Soil and Water Conservation

基　　金：国家自然科学基金项目(31070401);安徽省高校自然科学基金重大项目(KJ2018ZD033);安徽省高校自然科学基金重点项目(KJ2018A1000);重要生物资源保护与利用研究安徽省重点实验室基金项目

摘　　要：采用土培盆栽试验,研究了在铅污染土壤中添加叶绿醇对土壤酶活性和土壤铅离子有效态的影响,探讨了不同处理时间下叶绿醇对铅污染土壤的缓解作用。结果表明:短时间内在土壤中添加叶绿醇对土壤过氧化氢酶、蔗糖酶和碱性磷酸酶活性具有促进作用,对土壤脲酶活性具有抑制作用,且每种酶对叶绿醇的敏感程度不同,其中碱性磷酸酶最为敏感;随着处理时间延长土壤中过氧化氢酶活性、脲酶活性和铅离子有效态降低,蔗糖酶活性和碱性磷酸酶活性显著升高;土壤重金属铅浓度为600 mg/kg时,叶绿醇浓度为50 mg/kg蔗糖酶活性最强,比空白对照组高出122.28%;当土壤中叶绿醇浓度为250 mg/kg时,土壤碱性磷酸酶活性最高,是空白对照组的251.61%。总之,在铅污染的土壤中添加叶绿醇后能改变土壤酶活性,降低土壤中铅的有效态,并且随着培养时间的延长,叶绿醇对铅污染土壤的修复效果逐渐降低。A pot experiment was carried out to study the effects of applying phytol to lead(Pb)-contaminated soil on soil enzyme activity and Pb availability,and the alleviation effects of phytol to the Pb-contaminated soil under different treatment time were discussed.The results showed that adding phytol to soil in a short time promoted the activities of soil catalase,sucrase and alkaline phosphatase,while inhibited soil urease activity.Moreover,each enzyme had different sensitivity to phytol,among which alkaline phosphatase was the most sensitive.With the prolongation of treatment time,the activities of catalase and urease as well as available Pb content in the soil decreased,while the activities of invertase and phosphatase increased significantly.When the Pb concentration was 600 mg/kg in soil and the phytol concentration was 50 mg/kg,sucrase activity was the strongest,which was 122.28%higher than that of the blank control.Additionally,when the phytol concentration was 250 mg/kg in the soil,the activity of phosphatase was the strongest,which was 251.61%of the blank control.In conclusion,the addition of phytol to Pb-contaminated soil could change soil enzyme activity and reduce the content of available Pb in the soil.What’s more,with the prolongation of culture time,the alleviation effect of phytol on Pb-contaminated soil reduced gradually.

关 键 词：铅污染土壤 叶绿醇 土壤酶活性 有效态铅含量 

分 类 号：X173[环境科学与工程—环境科学] X53