哇巴因体外逆转肝癌耐药Bel-7402/ADM细胞的实验研究  

Experimental study on the reversal of hepatocarcinoma-resistant Bel-7402/ADM cells by Ouabain in vitro

作　　者：杨明镇[1] 赵逵[2] YANG Ming-zhen;ZHAO Kui(Department of Intervention,Affiliated Hospital of Zunyi Medical College,Guizhou Province,Zunyi563003,China;Department of Gastroenterology,Affiliated Hospital of Zunyi Medical College,Guizhou Province,Zunyi563003,China)

机构地区：[1]遵义医学院附属医院介入科,贵州遵义563003 [2]遵义医学院附属医院消化科,贵州遵义563003

出　　处：《中国当代医药》2019年第26期60-62,共3页China Modern Medicine

摘　　要：目的研究哇巴因体外逆转肝癌耐药Bel-7402/(阿霉素)ADM细胞机制。方法选用人肝癌Bel-7402细胞株,正常Bel-7402作为对照组,ADM大剂量间断冲击法建立Bel-7402/ADM模型作为耐药组,采用Real-Time-PCR检测哇巴因干预前后肿瘤多药耐药性(MDR-1)RNA表达,噻唑蓝溴化四唑(MTT)检测哇巴因耐药性并计算其耐药倍数、逆转倍数。结果耐药组对ADM耐药性显著高于对照组(P<0.05),耐药组耐药倍数显著高于对照组(P<0.05),哇巴因干预后对照组和耐药组IC50均显著低于干预前(P<0.05),耐药组逆转效果显著高于对照组(P<0.05),耐药组在哇巴因干预前后MDR-1RNA表达均显著高于对照组(P<0.05),干预后MDR-1RNA表达显著低于干预前(P<0.05)。结论哇巴因可通过降低MDR-1RNA表达体外逆转肝癌耐药Bel-7402/ADM细胞,值得临床推广,但哇巴因是否还参与其他耐药机制还待进一步研究。Objective To study the mechanism of Ouabain in reversing the hepatocarcinoma-resistant Bel-7402/Adriamycin(ADM)in vitro.Methods Human hepatoma Bel-7402 cell line was selected.A high-dose intermittent impact method was used to establish Bel-7402/ADM model as drug-resistant group and normal Bel-7402 was used as control group.Real-Time-PCR was used to detect tumor multidrug resistance(MDR-1)RNA expression before and after Ouabain intervention and Thiazolyl Blue tetrazolium bromide(MTT)was used to detect the effects of Ouabain resistance,calculating the drug resistance factor and reversal factor.Results The drug resistance of ADM was significantly higher in the drug-resistant group than that in the control group(P<0.05).The resistance factor in the drug-resistant group was significantly higher than that in the control group(P<0.05).IC50 in the control group and drug-resistant group after Ouabain intervention was decreased compared with pre-intervention(P<0.05),and the reversal effect of the drug-resistant group was significantly higher than that of the control group(P<0.05).The expression of MDR-1RNA in the drug-resistant group was significantly higher than that in the control group before Ouabain intervention(P<0.05);the expression of MDR-1RNA after intervention was significantly lower than that before intervention(P<0.05).Conclusion Ouabain reverses hepatocarcinoma-resistant Bel-7402/ADM cells in vitro by reducing MDR-1 RNA expression.

关 键 词：哇巴因 肝癌 耐药 阿霉素 

分 类 号：R735.7[医药卫生—肿瘤]