吕梁黑山羊成纤维细胞培养过程中霉菌污染的清除  

Removal of Mold Contamination of Lyuliang Black Goat Fibroblast during Cell Culture

作　　者：吴苏君[1] 王曦[1] 李希[1] 罗惠娣[1] 毛杨毅[1] 贺东昌[1] 周胜花[1] 李春艳[1] 张丽[1] 郭慧慧[1] 薛丽娜[1] 党文庆[1] WU Sujun;WANG Xi;LI Xi;LUO Huidi;MAO Yangyi;HE Dongchang;ZHOU Shenghua;LI Chunyan;ZHANG Li;GUO Huihui;XUE Lina;DANG Wenqing(Institute of Animal Husbandry and Veterinary,Shanxi Academy of Agricultural Sciences,Taiyuan 030032,China)

机构地区：[1]山西省农业科学院畜牧兽医研究所

出　　处：《山西农业科学》2019年第10期1834-1837,共4页Journal of Shanxi Agricultural Sciences

基　　金：国家绒毛用羊产业技术体系建设专项(CARS-39-24);山西省科技创新团队项目(201705D131028-20);山西省农业科学院科技自主创新能力提升工程项目(2017ZZCX-02)

摘　　要：为建立和完善细胞在体外培养过程中清除霉菌污染的方法,挽救珍贵的细胞资源,试验在被霉菌污染的吕梁黑山羊耳缘成纤维细胞培养液中分别添加2.5,25,250μg/mL的两性霉素B,每天洗涤、换液一次,连续处理14 d,比较不同浓度药物的清除效果。结果显示,细胞培养液中添加25μg/mL的两性霉素B后,细胞中无霉菌生长,细胞生长良好,药物处理过程中可正常传代,处理后细胞生长曲线与对照组细胞无明显差异。说明细胞培养液中添加25μg/mL两性霉素B既能清除霉菌污染又不会对细胞造成影响,该浓度是清除霉菌污染适宜的质量浓度。To establish and improve the method of removing mold contamination during cell culture in vitro,and to save precious cell resources,the experiment added 2.5,25,250μg/mL amphotericin B,respectively to the mold-cultured Lyuliang Black Goat ear fibroblast culture solution,washing and changing cell once a day,continuous treatment for 14 d,compared the removal effect of different concentrations of drugs.The results showed that after adding 25μg/mL amphotericin B to the cell culture medium,there was no mold growth in the cells,and the cells grew well.The cells were normally passaged during the drug treatment.There was no significant difference between the cell growth curve and the control cells.Adding 25μg/mL amphotericin B to the cell culture solution can eliminate mold contamination without affecting the cells,and this concentration is an appropriate concentration for removing mold contamination.

关 键 词：吕梁黑山羊 细胞培养 霉菌污染 霉菌清除 两性霉素B 

分 类 号：S827[农业科学—畜牧学]