拟松材线虫纤维素酶活性与其致病性关系  

Studies on correlation between cellulase activity of Bursaphelenchus mucronatus and its pathogenicity

作　　者：薛美静 李英 张靖 高泽文[1] 吴文涛 王扬[1] 喻盛甫[1] XUE Meijing;LI Ying;ZHANG Jing;GAO Zewen;WU Wentao;WANG Yang;YU Shengfu(College of Plant Protection,Yunnan Agriculture University,Kunming,Yunnan 650201,China)

机构地区：[1]云南农业大学植物保护学院

出　　处：《生物安全学报》2019年第3期181-188,共8页Journal of biosafety

基　　金：国家重点研发计划(2016YFC1202104)

摘　　要：【目的】研究拟松材线虫是否分泌纤维素酶以及纤维素酶与其致病力的关系。【方法】对拟松材线虫的不同致病性种群以及松材线虫的虫体蛋白提取液、分泌液的纤维素酶活性进行定量测定,比较群体间纤维素同工酶谱型差异。【结果】拟松材线虫也含有纤维素酶,并向体外分泌;且不同致病性种群的纤维素酶活性与其致病性有一定相关性,致病性越强纤维素酶活性越强。【结论】纤维素酶活性大小是导致拟松材线虫不同种群间致病性差异的重要原因,这对于全面认识松树线虫萎蔫病的致病机理有重要意义。【Aim】To study whether Bursaphelenchus mucronatus secretes cellulase and whether this cellulase is related to its pathogenicity is not clear.【Method】We measured the cellulase activity of the protein extraction and secretion of two pathogenic species,B.mucronatus and B.xylophilus,and compared the differences in the isoforms of cellulose isozymes between the populations.【Result】Both the protein extraction and secretion of B.mucronatus contained cellulase.And there were some relativity between the cellulose activity and the pathogenicity of B.mucronatus,0x09the stronger the pathogenicity,the stronger the cellulase activity.【Conclusion】Cellulase activity is an important reason for the difference in pathogenicity between different populations of B.mucronatus,which is of great significance for comprehensive understanding of the pathogenic mechanism of pine wilt disease.

关 键 词：拟松材线虫 纤维素酶 致病性 同工酶 

分 类 号：S85[农业科学—兽医学]