甘蓝型油菜发芽期低温耐性的评价与材料筛选  

Screening and evaluation of low temperature tolerance of rapeseed (Brassica napus L.) at germination stage

作　　者：黄贺 闫蕾 吕艳 丁晓雨 蔡俊松 程勇[1] 张学昆[1] 邹锡玲[1] HUANG He;YAN Lei;LYU Yan;DING Xiao-yu;CAI Jun-song;CHENG Yong;ZHANG Xue-kun;ZOU Xi-ling(Oil Crops Research Institute of Chinese Academy of Agricultural Sciences,Key Laboratory Biology and Genetic Im provement of Oil Crops,Ministry of Agriculture and Rural Affairs,Wuhan 430062;Hubei Province Oilseed Rape Of fice,Wuhan 430060,China)

机构地区：[1]中国农业科学院油料作物研究所/农业农村部油料作物生物学与遗传育种重点实验室,湖北武汉430062 [2]湖北省油菜办公室,湖北武汉430060

出　　处：《中国油料作物学报》2019年第5期723-734,共12页Chinese Journal of Oil Crop Sciences

基　　金：国家重点研发计划(2017YFD0101700);中国农业科学院农业科技创新计划;湖北省农业科技创新中心

摘　　要：为建立甘蓝型油菜发芽期低温耐性的鉴定及评价方法,筛选强耐低温材料,分别在22℃和9℃对66份材料进行发芽势、发芽率、发芽指数以及平均发芽时间的考察,利用隶属函数法进行综合评价,并通过聚类分析划分材料的低温耐性等级。结果表明:9℃低温会降低发芽势、发芽率和发芽指数,延长发芽时间。不同类型的品种间耐低温程度差异明显:中部品种的耐低温能力高于北部品种,半冬性性品种的耐低温能力高于春性品种与冬性品种,常规种的耐低温能力高于杂交种。单一指标与综合隶属函数值对品种的耐低温评价结果基本一致。筛选出极端耐低温品种H5(秦油8号×e6013)和敏感品种S10(汇丰一号)。To establish evaluation method for low temperature tolerance of rapeseed(Brassica napus L.)at ger mination stage,66 rapeseed lines were investigated under the conditions of 22°C and 9°C.The parameters including germination potential,germination rate,germination index and mean germination time were investigated.After eval uation,materials were categorized by cluster analysis into different low temperature tolerance grades.The results showed that 9°C(low temperature)decreased germination potential,germination rate and germination index,it also prolonged germination time.The materials’temperature tolerances were obvious among types:materials from mid land were more tolerant than those from north,semi-winter-type lines were more tolerant than spring-and wintertype materials,conventional varieties were more tolerant than hybrids.The evaluation results from single indicators were consistent with those from comprehensive membership values.Among the 66 materials,H5(hybrid of Qinyou 8×e6013)was the extremely low-temperature-resistant variety,and S10(Huifeng 1)was the sensitive one.

关 键 词：油菜 萌发 耐低温 隶属函数 聚类分析 

分 类 号：S565.4[农业科学—作物学] S332.5