Beclin1与凋亡相关蛋白在口腔鳞癌中的表达及意义  

Expression and Significance of Beclin1 and Apoptosis Related Proteins in Oral Carcinogenesis

作　　者：孙维克[1] 王培源[1] 刘伟[1] 王霞[1] SUN Weike;WANG Peiyuan;LIU Wei;WANG Xia(Binzhou Medical University,Yantai 264000,China)

机构地区：[1]滨州医学院

出　　处：《口腔医学研究》2019年第10期979-983,共5页Journal of Oral Science Research

摘　　要：目的:探讨Beclin1及凋亡相关蛋白bcl-2、survivin及bax在口腔鳞癌中的表达及临床意义。方法:采用免疫组化SP法检测口腔黏膜癌变组织中(包括鳞癌60例,异常增生48例,癌旁正常口腔黏膜60例)Beclin1、bcl-2、survivin及bax的表达,分析其相关性及临床意义。结果:Beclin1在鳞癌和正常黏膜呈高水平表达(表达阳性率分别为65%,75%),在异常增生组的表达显著性降低(54.2%,P<0.05);bcl-2及survivin在鳞癌组织中表达水平(70%,73.33%)显著高于异常增生和正常黏膜(P<0.05),bax则在异常增生组表达显著高于鳞癌和正常黏膜(P<0.05)。口腔癌组织Beclin1的表达与bcl-2、survivin、bax的表达均密切相关(P<0.05;相关系数分别为r=0.421,r=0.367,r=0.542),四者均与肿瘤大小及颈部淋巴结转移密切相关。结论:Beclin1与凋亡相关蛋白在口腔鳞癌的癌变过程中密切相关,自噬与凋亡可能协同参与口腔癌的进展。Objective:To study the clinical significance of Beclin1 and its relationship with bcl-2,survivin,and bax in oral carcinogenesis.Methods:Immunohistochemical SP method was used to detect the expression of Beclin1,bcl-2,survivin,and bax in oral carcinogenesis tissues including oral cancer(60 cases),oral dysplasia(48 cases),and normal oral mucosa(60 cases),and to study the clinical significance of Beclin 1 in oral squamous cell carcinoma(OSCC)and its relationship with bcl-2,survivin,and bax expression in oral carcinogenesis.Results:The expression of Beclin1 was significantly increased in OSCC and normal mucosa(65%and 75%,respectively),and the positive expression rate in dysplasia significantly decreased(54.2%,P<0.05).Survivin and bcl-2 expression were higher in OSCC(73.33%and 70%)than in dysplasia and normal mucosa(P<0.05);the expression rate of bax increased in dysplasia than OSCC and normal mucosa(P<0.05).Beclin1,bcl-2,survivin,and bax expression was closely related to diameter of tumor and lymph node metastasis(P<0.05).The expression of Beclin1 was closely related to bcl-2,survivin,and bax(P<0.05;r=0.421,0.367 and 0.542)in OSCC.Conclusion:Autophagy and apoptosis were involved in oral carcinogenesis,and were associated with invasion and metastasis of oral cancer,which synergistically participated in the development of oral cancer.

关 键 词：BECLIN1 自噬 凋亡 口腔癌 

分 类 号：R73[医药卫生—肿瘤]