300 mM高渗盐溶液对重度烫伤大鼠肾损伤的影响  

Influence of 300 mM Hypertonic Saline on the Renal Injury of Severely Scalded Rats

作　　者：王彪[1] 袁春雨[1] 王沁澄 田播文 孙业祥[1] Wang Biao;Yuan Chunyu;Wang Qincheng;Tian Bowen;Sun Yexiang(Burn Surgery Department of The First Affiliated Hospital of Anhui Medical University,Hefei City,Anhui 230022,China)

机构地区：[1]安徽医科大学第一附属医院烧伤科

出　　处：《中国烧伤创疡杂志》2019年第5期325-329,共5页The Chinese Journal of Burns Wounds & Surface Ulcers

基　　金：国家自然科学基金(81272092)~~

摘　　要：目的探讨300 mmol/L(mM)高渗盐溶液(HS)对重度烫伤大鼠肾损伤的影响。方法按照随机数表法将56只大鼠随机分为假伤组(8只)、LR组(24只)与HS组(24只),其中假伤组大鼠做假伤处理后直接处死,LR组大鼠建立重度烫伤模型后予以乳酸钠林格注射液(LR)复苏治疗,HS组大鼠建立重度烫伤模型后予以300 mM HS(1 L 300 mM HS含97 mL 10%NaCl和903 mL LR)复苏治疗,对比观察各组大鼠肾脏组织湿干重比值(W/D)、p38丝裂原活化蛋白激酶(p38 mitogen activated protein kinases,p38 MAPK)通道活化程度及血清肌酐(Cr)、中性粒细胞明胶酶相关脂质运载蛋白(neutrophil gelatinase associated lipocalin,NGAL)、肿瘤坏死因子 α(TNF α)、白细胞介素 6(IL 6)、高迁移率族蛋白B1(highmobility group box1,HMGB1)的水平变化。结果与假伤组对比,LR组及HS组大鼠伤后2、8、24 h肾脏组织W/D、p38 MAPK通道活化程度及血清Cr、NGAL、TNF α、IL 6水平,以及伤后8、24 h HMGB1水平均较高,P均<0.05,差异具有统计学意义;而伤后2 h HMGB1水平与假伤组相同,P>0.05,差异无统计学意义。与LR组对比,HS组大鼠伤后2、8、24 h肾脏组织W/D、p38 MAPK通道活化程度均较低,P均<0.05,差异具有统计学意义;HS组大鼠伤后2 h血清Cr、HMGB1水平与LR组基本相同,P均>0.05,差异无统计学意义,而伤后8、24 h血清Cr、HMGB1水平均低于LR组,P均<0.05,差异具有统计学意义;HS组大鼠伤后2、8、24 h血清NGAL、TNF α、IL 6水平均低于LR组,P均<0.05,差异具有统计学意义。结论HS可通过抑制血清炎症介质的产生及下调p38 MAPK信号通路活化程度而减轻肾脏的损伤程度,故烧伤后可早期应用HS予以复苏抗休克。Objective To explore the influence of 300 mmol/L(mM)hypertonic saline(HS)on the renal injury of severely scalded rats.Methods Fifty-six rats were randomly divided into a sham group(8 rats),LR group(24 rats)and HS group(24 rats)according to the random number table.In the sham group,rats were killed directly after fake injury management,rats in the LR group were injected sodium lactate ringer’s injection(LR)for resuscitation after the establishment of severe scalds,while rats in the HS group were given 300 mM HS(1 L of 300 mM HS containing 97 mL 10% NaCl and 903 mL LR)for resuscitation after the establishment of severe scalds.In the three groups,the wet/dry weight ratio(W/D)of renal tissues,the activation degree of p38 mitogen-activated protein kinases(p38 MAPK)channel,and the expression levels of serum creatinine(Cr),neutrophil gelatinase-associated lipocalin(NGAL),tumor necrosis factor-a(TNF-a),interleukin-6(IL-6)and high mobility group box 1(HMGB1)were respectively observed and compared.Results Compared with the sham group,the W/D,activation degree of p38 MAPK channel and the expression levels of serum Cr,NGAL,TNF-α and IL-6 at 2 h,8 h and 24 h after scalds,and the expression levels of HMGB1 at 8 h and 24 h after scalds in the LR group and HS group were all improved,and the differences all showed statistical significance(all P<0.05),while the expression level of HMGB1 at 2 h after scalds was same as that in the sham group,presenting no statistically significant difference,P>0.05.The W/D and the activation degree of p38 MAPK channel in renal tissues in the HS group at 2 h,8 h and 24 h after scalds were lower as compared with that in the LR group,and the differences were statistically significant,all P<0.05.In the HS group,the expression levels of serum Cr and HMGB1 at 2 h after scalds were basically the same as that in the LR group,and the differences showed no statistical significance(P>0.05),while the expression levels of serum Cr and HMGB1 at 8 h and 24 h after scalds were lower than that in the LR group,an

关 键 词：烫伤 高渗盐溶液 肾损伤 炎症介质 P38丝裂原活化蛋白激酶 影响 

分 类 号：R73[医药卫生—肿瘤]