来源于印度洋深海区的烟曲霉次生代谢产物的研究  

Study of the secondary metabolites of Aspergillus fumigatus from the deepsea zone of the Indian Ocean

作　　者：李冬艳 王晶[1] 陈科良 朱虎成[2] 陈春梅[2] 张锦文[1] LI Dong-yan;WANG Jing;CHEN Ke-liang;ZHU Hu-cheng;CHEN Chun-mei;ZHANG Jin-wen(Department of Pharmacy,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China;Pharmaceutical college,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China)

机构地区：[1]华中科技大学同济医学院附属同济医院药学部,湖北武汉430030 [2]华中科技大学同济医学院药学院,湖北武汉430030

出　　处：《海洋科学》2019年第8期43-49,共7页Marine Sciences

基　　金：国家自然科学基金青年项目(31600266)~~

摘　　要：为了研究烟曲霉次生代谢产物,我们采用硅胶柱层析、sephadex LH-20凝胶柱层析、半制备液相色谱等分离方法对来源于印度洋深海区的烟曲霉(Aspergillus fumigatus)的乙醇提取液进行系统的次生代谢产物分离,根据所分离得到的化合物的理化性质和波谱数据确定化合物的结构。共分离鉴定了15个化合物,其中11个二酮哌嗪类生物碱(diketopiperazine),4个喹唑啉类衍生物(fumiquinazoline),分别为:fumitremorgin B(1),13-oxofumitremorgin B(2),fumitremorgin C(3),12-hydroxyfumitremorgin C(4),cyclotyprostatins B(5),cyclotyprostatins C(6),cyclotyprostatins E(7),verruculogen(8),spirotry?prostatin C(9),spirotryprostatin D(10),spirotryprostatin F(11),fumiquinazoline C(12),fumiquinazoline D(13),3-hydroxyfumiquinazoline A(14)和fumiquinazoline E(15)。分离得到的15个化合物,丰富了烟曲霉次生代谢产物的类型,为烟曲霉的开发利用提供了化学研究基础。This study was conducted to analyze the chemical constituents of the secondary metabolites of Aspergillus fumigatus for which the chemical constituents of the ethanol extract of A.fumigatus from the deep sea zone of the In-dian Ocean were separated systematically using silica gel column chromatography,Sephadex LH-20 column chroma-tography,and RP-HPLC.Physicochemical properties and spectral data were used to determine the structures of the iso-lated compounds.A total of 15 compounds were isolated that were identified as fumitremorgin B(1),13-oxofumitremorgin B(2),fumitremorgin C(3),12-hydroxyfumitremorgin C(4),cyclotyprostatins B(5),cyclotypros-tatins C(6),cyclotyprostatins E(7),verruculogen(8),spirotryprostatin C(9),spirotryprostatin D(10),spirotryprostatin F(11),fumiquinazoline C(12),fumiquinazoline D(13),3-hydroxyfumiquinazoline A(14),and fumiquinazoline E(15).These 15 compounds isolated from A.fumigatus enriched the type of the secondary metabolites of the fungus and pro-vided a basis for exploring its development and conducting chemical research.

关 键 词：烟曲霉(Aspergillus fumigatus) 曲霉属(Aspergillus) 次生代谢产物 化学成分 波谱数据 

分 类 号：R914.4[医药卫生—药物化学]