高脂日粮对肥胖易感和肥胖抵抗小鼠骨性能的影响以及槲皮素的干预作用  

Effects of High-Fat Diet on the Bone of Obesity Prone and Obesity Resistant Mice and the Intervention of Quercetin

作　　者：周银芝 夏淑芳[1] 段晓梅[1] 颜彪 施用晖[1,2] 乐国伟[1,2] ZHOU Yinzhi;XIA Shufang;DUAN Xiaomei;YAN Biao;SHI Yonghui;LE Guowe(School of Food Science and Technology,Jiangnan University,Wuxi 214122,China;State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学食品学院,江苏无锡214122 [2]食品科学与技术国家重点实验室,江南大学,江苏无锡214122

出　　处：《食品与生物技术学报》2019年第7期100-107,共8页Journal of Food Science and Biotechnology

基　　金：国家“十二五”科技支撑计划项目（2012BAD33B05）

摘　　要：探究饲喂高脂日粮对肥胖易感(obesity prone,OP)和肥胖抵抗(obesity resistant,OR)小鼠骨性能的影响及槲皮素在其中的干预作用。将小鼠随机分为正常日粮组(C)和高脂日粮组。7 w后,将高脂日粮组的小鼠按体质量分出OR组和OP组,并在OP组和OR组小鼠的高脂日粮中添加槲皮素进行干预,分别测定槲皮素添加前后各组小鼠的股骨性能参数、氧化还原状态以及成骨细胞和破骨细胞分化相关基因的表达。结果显示,高脂日粮显著降低OP和OR小鼠的骨性能,显著提高破骨细胞分化相关基因的表达,显著降低成骨细胞分化相关基因的表达,加入槲皮素后能得到恢复。与前面结果一致,高脂日粮显著降低OP和OR小鼠股骨的抗氧化指标以及相关基因的表达,显著升高股骨MDA质量摩尔浓度,加入槲皮素后均能得到恢复。因此高脂日粮导致肥胖易感和肥胖抵抗小鼠股骨氧化应激,可能影响成骨细胞和破骨细胞分化相关基因的表达降低骨性能,槲皮素可通过改善两种小鼠股骨的氧化还原状态提高骨性能。To investigate the effects of high-fat diet(HFD)on the bone of obesity resistant(OR)and obesity prone(OP)mice and the intervention of quercetin.Mice were randomly divided into two groups:regular diet group(C)and HFD group.After 7 weeks,HFD-fed mice were devided into OP and OR group according to body weight and treated with quercetin.Femur parameters,redox status and expressions of osteoblast and osteoclast differentiation related genes were measured before and after quercetin treatment.The results showed that HFD treatment significantly decreased femur parameters of OP and OR mice.The expressions of osteoclast differentiation related genes were significantly up-regulated while the osteoblast differentiation related genes were down-regulated significantly in both OP and OR mice.Femur properties were improved after quercetin treatment.Consistent with the above mentioned,the content of MDA was higher,while the antioxidant indexes and the expression of antioxidant related genes were significantly lower in OP and OR mice.Redox status was improved after quercetin treatment.HFD treatment led to oxidative stress in femurs of OR and OP mice,may by affecting the expressions of osteoblast and osteoclast differentiation related genes,consequently reduced the femur properties.Quercetin can improve redox status,thereby improving femur properties of HFD-fed OP and OR mice.

关 键 词：高脂日粮 肥胖易感 肥胖抵抗 骨性能 氧化还原状态 

分 类 号：R151.2[医药卫生—营养与食品卫生学]