金纳米棒介导近红外光热疗杀伤胃癌SGC-7901细胞及诱导凋亡的实验  

Antitumor and Apoptosis Induction Effects of Gold Nanorods-mediated Near-infrared Photothermal Therapy on Gastric Cancer SGC-7901 Cells in vitro

作　　者：陈志奎[1] 杨菁[2] 谢丽红 CHEN Zhikui;YANG Jing;XIE Lihong(Department of Ultrasound,Affiliated Union Hospital of Fujian Medical University,Fuzhou 350001,China;Department of Pharmacy,Affiliated Union Hospital of Fujian Medical University,Fuzhou 350001,China)

机构地区：[1]福建医科大学附属协和医院超声科,福州350001 [2]福建医科大学附属协和医院药学部,福州350001

出　　处：《肿瘤防治研究》2019年第10期890-894,共5页Cancer Research on Prevention and Treatment

基　　金：福建省卫生系统中青年骨干人才培养重点项目(2015-ZQN-ZD-13)

摘　　要：目的探讨不同剂量的金纳米棒和近红外光照射对体外培养的胃癌SGC-7901细胞的杀伤效能。方法采用晶种生长法制备金纳米棒,并对其形貌、粒径和光吸收峰进行检测。采用CCK-8法检测金纳米棒对体外培养的SGC-7901细胞增殖的抑制作用;检测不同浓度金纳米棒、不同功率近红外光照射对胃癌细胞增殖的抑制作用。采用Annexin V-FITC/PI双染法分析金纳米棒介导近红外光热疗对胃癌SGC-7901细胞凋亡的诱导作用,采用分光光度法检测Caspase-3、Caspase-9酶活性变化。结果合成金纳米棒的纵向等离子共振吸收峰位于790 nm。单纯金纳米棒孵育对胃癌SCT7901细胞毒性较低,但金纳米棒联合近红外光照射热疗具有明显杀伤胃癌细胞作用,随着金纳米棒剂量和近红外光功率的升高,细胞生长抑制率明显上升,40μg/ml的金纳米棒,3.0 W/cm2近红外光照射2 min,1 h后细胞增殖抑制率即达到93.4%。金纳米棒介导近红外光热疗后1 h,部分细胞处于早期凋亡状态,而3 h时细胞进入晚期凋亡及死亡状态,Caspase-3、Caspase-9酶活性均升高约1倍。结论金纳米棒介导近红外光热疗具有明显杀伤胃癌细胞作用,并且可诱导胃癌细胞凋亡。Objective To investigate the antitumor efficacy of near-infrared photothermal therapy(NIPT)at different doses of gold nanorods(GNRs)and near-infrared light on gastric cancer SGC-7901 cells in vitro.Methods GNRs were prepared by seed-mediated growth method,and their morphology,particle size and light absorption were detected.The inhibitory rates of GNRs on the proliferation of SGC-7901 cells were measured by CCK-8.The inhibitory rates of GNRs at different concentrations and NIPT at different powers on the proliferation of SGC-7901 cells were calculated.Meanwhile,the apoptosis of gastric cancer cells was detected by Annexin V-FITC/PI fluorescence method,and the Caspase-3 and Caspase-9 enzyme activities were detected by spectrophotometry.Results The synthesized GNRs had a longitudinal plasmon resonance absorption peak at around 790nm.The cytotoxicity of pure GNRs was really low.However,NIPT had obvious antitumor effects on gastric cancer cells.With the increase of GNRs dose and near-infrared light power,the cell growth inhibition rate increased significantly.After administration of 40μg/ml GNRs for 1h and 3.0W/cm2 near-infrared radiation for 2min,the cell growth inhibition rate reached 93.4%.One hour after treatment,part of the cells were in early apoptotic stage;while three hours after treatment,the cells were in advanced apoptosis or death state,and the Caspase-3,Caspase-9 enzyme activity increased about one fold,compared with those of control group.Conclusion GNRs-mediated NIPT has obvious antitumor and apoptosis induction effects on gastric cancer cells.

关 键 词：近红外光 金纳米棒 晶种生长法 细胞生长抑制率 细胞凋亡 

分 类 号：R73-36[医药卫生—肿瘤] R735.2[医药卫生—临床医学]