大肠癌血瘀证与非血瘀证患者肿瘤组织血管新生标记物、HIF-1α和VEGF表达的差异性  

Differences on expressions of angiogenenic markers, HIF-1α and VEGF in tumor tissue of colorectal cancer patients with blood stasis syndrome or non-blood stasis syndrome

作　　者：周利红[1,2] 王婷 韩植芬[1] 伏杰 刘宣[1] 冯媛媛[1] 隋华[1] 季青[1] 王炎[1] 李雁[2] 李琦[1] ZHOU Lihong;WANG Ting;HAN Zhifen;FU Jie;LIU Xuan;FENG Yuanyuan;SUI Hua;JI Qing;WANG Yan;LI Yan;LI Qi(Department of Oncology,Cancer Institute,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Department of Oncology,Shanghai Municipal Hospital of Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200071,China)

机构地区：[1]上海中医药大学附属曙光医院肿瘤科、肿瘤研究所,上海201203 [2]上海中医药大学附属市中医医院肿瘤科,上海200071

出　　处：《上海中医药杂志》2019年第10期28-32,共5页Shanghai Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金重点项目(81830120);国家自然科学基金面上项目(81673784,81673783);国家自然科学基金青年基金项目(81703893);上海市科委青年科技启明星项目(18QA1404200,17QA1414100);上海市卫计委进一步加快中医药事业发展三年行动计划项目[ZY(2018-2020)-FWTX-4026];上海中医药大学杏林青年学者项目(2017年)

摘　　要：目的通过检测大肠癌血瘀证与非血瘀证患者肿瘤组织的血管新生标记物、缺氧诱导因子-1α(HIF-1α)和血管内皮生长因子(VEGF)的表达情况,分析大肠癌血瘀证与肿瘤血管新生之间的关系。方法将116例(均为回顾性病例)临床大肠癌患者分为血瘀证组与非血瘀证组,采用免疫组织化学技术检测肿瘤组织中的血管新生肿瘤标记和HIF-1α和VEGF的阳性表达情况,结合临床分期分析大肠癌血瘀证与非血瘀证患者上述指标表达的差异性。结果①大肠癌血瘀证与非血瘀证患者的临床分期以Ⅱa期和Ⅲb期为主,差异无统计学意义(P>0.05);②大肠癌血瘀证与非血瘀证患者肿瘤组织血管CD34的阳性表达差异有统计学意义(P<0.001),CK-7、CK-20、CEA、CDX-2、p53、Ki-67、神经S-100、淋巴管D2-40的阳性表达差异无统计学意义(P>0.05);③大肠癌血瘀证患者肿瘤组织HIF-1α和VEGF的阳性表达高于大肠癌非血瘀证患者(P<0.001)。结论大肠癌血瘀证患者肿瘤血管CD34、HIF-1α和VEGF的阳性表达明显高于非血瘀证患者,提示大肠癌血瘀证与肿瘤血管新生密切相关。Objective To detect the expression levels of angiogenic tumor markers,hypoxia inducible factor-1α( HIF-1α) and vascular endothelial growth factor( VEGF) in tumor tissue of colorectal cancer patients with blood stasis syndrome or non-blood stasis syndrome,and analysis the relationship between blood stasis syndrome and tumor angiogenesis. Methods One hundred and sixteen patients( retrospective cases) were divided into the blood stasis syndrome group and non-blood stasis syndrome group. The positive expressions of angiogenic tumor markers,HIF-1α and VEGF in tumor tissue were measured by immunohistochemistry,and combined with clinical stages,the differences on the expressions of above indicators in colorectal cancer patients with blood stasis syndrome or non-blood stasis syndrome were analyzed. Results(1) The clinical stages of colorectal cancer patients with blood stasis syndrome or non-blood stasis syndrome were mainly stage Ⅱa and stage Ⅲb,and there was no statistical significance between the two groups( P > 0. 05).(2) There was statistically significant difference on the positive expression of vascular CD34 in tumor tissue between colorectal cancer patients with blood stasis syndrome and those patients with non-blood stasis syndrome( P<0.001), but there were no statistically significant differences on the positive expressions of CK-7,CK-20,CEA,CDX-2,p53,Ki-67,nerve S-100 and lymphatic D2-40( P>0.05).(3) The positive expressions of HIF-1α and VEGF in tumor tissue of colorectal cancer patients with blood stasis syndrome were significantly higher than those of colorectal cancer patients with non-blood stasis syndrome( P<0.001). Conclusion Compared with colorectal cancer patients with non-blood stasis syndrome,the positive expressions of CD34,HIF-1α and VEGF in tumor are significantly increased in patients with blood stasis syndrome,which suggests that blood stasis syndrome of colorectal cancer is closely related to tumor angiogenesis.

关 键 词：大肠癌 血瘀证 血管新生 缺氧诱导因子-1Α 血管内皮生长因子 

分 类 号：R73[医药卫生—肿瘤]