高产木质素降解酶菌株的筛选及其生物学特性研究  

A Study on Ligninolytic Enzyme-producing Fungi

作　　者：袁海华 张保[1] 张颖 敖新宇[1] YUAN Hai-hua;ZHANG Bao;ZHANG Ying;AO Xin-yu(College of Life Science,Southwest Forestry University,Kunming,Yunnan650224,China;College of Biodiversity Conservation and Utilization,Southwest Forestry University,Kunming,Yunnan650224,China)

机构地区：[1]西南林业大学生命科学院,云南昆明650224 [2]西南林业大学生物多样性保护与利用学院,云南昆明650224

出　　处：《福建农业学报》2019年第7期829-836,共8页Fujian Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(31560013)

摘　　要：【目的】获得高产木质素降解酶(LMLs,包括漆酶Lac、锰过氧化物酶MnP、木质素过氧化物酶LiP)的菌株,为木腐菌菌种资源的合理开发和应用提供一定理论依据。【方法】对9株具有产LMLs能力的木腐菌进行产酶筛选,并对筛选出的菌株的生物学特性进行研究。【结果】结果表明:在9株木腐菌中,产LMLs能力较高的菌株分别为LS136(Lac:0.18 U·L^-1、LiP:109.68 U·L^-1、MnP:140.38 U·L^-1)、LJ485(Lac:136.99 U·L^-1、LiP:9.53 U·L^-1、MnP:72.05 U·L^-1)和LJ496(Lac:163.39 U·L^-1、LiP:9.50 U·L^-1、MnP:74.36 U·L^-1)。生物学特性研究结果显示3种菌株最适生长温度均为25℃左右,适宜的生长pH值在4~8。此外,3种菌株对0~8 mmol·L^-1的重金属Cr 3+、Pb 2+以及0~4 mmol·L^-1的Cu 2+均有较强的耐受性。当Cr 3+为1~4 mmol·L^-1时,对LJ485和LJ496的生长具有一定的促进作用。【结论】菌株LS136、LJ485和LJ496分别属于韧革菌属Stereum sp.彩绒革盖菌Trametes versicolor和小薄孔菌Antrodiella sp.,具有较强的产LMLs能力和一定的重金属耐受性,为降解木质素的菌株开发提供了菌种资源参考。【Objective】To search for wood-rotting fungi that are highly effective in producing ligninolytic enzymes(LMLs),such asLac,MnP,and LiP.【Methods】Nine strains of wood-rotting fungi with known ability to produce LMLs were screened and their biological characteristics studied.【Results】The strains with high capacity of generating LMLs and their yields on each of the LMLs were found to be:LS136(yielding Lac at 0.18 U·L^-1,LiP at 109.68 U·L^-1,and MnP at 140.38 U·L^-1),LJ485(yielding Lac at 136.99 U·L^-1,LiP at 9.53 U·L^-1,and MnP at 72.05 U·L^-1),and LJ496(yielding Lac at 163.39 U·L^-1,LiP at 9.50 U·L^-1,and MnP at 74.36 U·L^-1).The optimum growth temperature for the 3 fungal strains was approximately 25℃,and pH ranged 4 to 8.The strains were all highly tolerant to Cr 3+or Pb 2+at concentrations of 0-8 mmoL·L^-1 and Cu 2+at 0-4 mmoL·L^-1.At the concentrations between 1-4 mmoL·L^-1,Cr 3+promoted the growth of LJ485 and LJ496.【Conclusion】The identified LS136 was thought to be a Stereum sp.,LJ485 identified as Trametes versicolor,and LJ496 considered an Antrodiella sp..They all demonstrated varying capacities of producing LMLs with a tolerance to heavy metals.The information would be useful for further investigation on the utilization of these lignin degrading fungi.

关 键 词：木腐菌 木质素降解酶 生物学特性 

分 类 号：Q93[生物学—微生物学]