一起鼠伤寒沙门氏菌食物中毒的病原鉴定与同源性分析  

Pathogen Identification and Homology Analysis of A Salmonella Typhimurium Outbreak

作　　者：肖波[1] 陶顺 徐洪琳 李淋 陈曦[1] XIAO Bo;TAO Shun;XU Honglin;LI Lin;CHEN Xi(Zigong Muncipal Center for Disease Control and Prevention,Zigong 643000,Sichuan Province,China)

机构地区：[1]自贡市疾病预防控制中心,四川自贡643000 [2]荣县疾病预防控制中心,四川自贡643100

出　　处：《职业卫生与病伤》2019年第5期319-322,共4页Occupational Health and Damage

摘　　要：目的对发生在自贡市某县的一起食物中毒事件进行病原分离鉴定及同源性分析。方法按照GB4789.4-2016和WS271-2007进行病原菌的分离鉴定。采用脉冲场凝胶电泳(PFGE)技术对分离到的鼠伤寒沙门氏菌进行分子分型。结果在1份粪便样本,1份患者肛拭子样本和6份食物样本中检出鼠伤寒沙门氏菌;分离到的8株鼠伤寒沙门氏菌经脉冲场凝胶电泳得到同一种带型,图谱相似度为100%。结论结合流行病学调查资料、临床诊断,确认此次食物中毒由同一克隆鼠伤寒沙门氏菌污染所致,PFGE能够在食物中毒事件的追踪溯源中发挥重要作用。Objective To identify the pathogen and make homology analysis of a foodborne disease outbreak in a county of Zigong city.Methods The pathogen was isolated and identified based on the GB 4789.4-2016 and WS 271-2007 standard,and the molecular subtyping was performed by pulsed field gel electrophoresis(PFGE)of the isolated Salmonella Typhimurium strains.Results The salmonella typhimurium was isolated from 1 stool and 1 anal swab samples of the patients,and 6 food samples.The 8 isolated Salmonella Typhimurium strains were 100%identical according to PFGE analysis.Conclusion This outbreak was confirmed was caused by food pollution of a single strain of salmonella typhimurium combined with epidemiological evidence and clinical diagnosis.The PFGE could be a very powerful tool in tracing the source of foodborne disease outbreaks.

关 键 词：鼠伤寒沙门氏菌 病原鉴定 脉冲场凝胶电泳 

分 类 号：R155.3[医药卫生—营养与食品卫生学] R446.5[医药卫生—公共卫生与预防医学]