长链非编码RNA-ROR对结直肠癌SW620细胞增殖的影响  

Effect of LncRNA-ROR on the proliferation of colorectal cancer SW620 cells

作　　者：施炜[1] 胡世丰[1] 雷春[2] 吴伙 SHI Wei;HU Shi-feng;LEI Chun;WU Huo(Department of Medical Morphology,Department of Medicine,Tongling Vocational and Technical College,Tongling Anhui 244061;Department of General Surgery,Tongling People′s Hospital,Tongling Anhui 244000;Department of Hepatobiliary Surgery,The First Affiliated Hospital of Anhui Medical University(High-tech Institute),Hefei Anhui 230088,China)

机构地区：[1]铜陵职业技术学院医学系基础形态教研室,安徽铜陵244061 [2]安徽省铜陵市人民医院普外科,244000 [3]安徽医科大学第一附属医院(高新院区)肝胆外科,安徽合肥230088

出　　处：《蚌埠医学院学报》2019年第10期1297-1300,共4页Journal of Bengbu Medical College

基　　金：安徽省教育厅高校自然科学研究重点项目(KJ2017A635)

摘　　要：目的:了解长链非编码RNA-ROR(LncRNA-ROR)在结直肠癌SW620细胞增殖中的作用。方法:选择40例结直肠癌组织及相应正常组织(距肿瘤切缘5 cm以外),首先采用qRT-PCR技术检测LncRNA-ROR在结直肠癌组织中的表达情况,并分析其表达与病人临床病理因素的相关性。构建慢病毒载体LV5-ROR、LV5-Vector转染到结直肠癌细胞SW620中,验证LncRNA-ROR的表达,分析LncRNA-ROR过表达对结直肠癌细胞CCK-8功能及SW620平板克隆形成的影响。结果:与正常组织比较,LncRNA-ROR在结直肠癌组织中低表达(P<0.01),其表达水平与TNM分期具有相关性(P<0.05)。经转染筛选后,qRT-PCR检测证实SW620中的LncRNA-ROR表达增加,其高表达导致SW620增殖能力的下降(P<0.01)。结论:LncRNA-ROR的过表达抑制了结直肠癌细胞SW620的增殖。Objective:To investigate the effects of long non-coding RNA-ROR(LncRNA-ROR)on the proliferation of colorectal cancer SW620 cells.Methods:The expression level of LncRNA-ROR in colorectal cancer tissue was detected using qRT-PCR,and the relationship between the expression level of LncRNA-ROR and clinicopathological data was analyzed.The lentiviral vector LV5-ROR was constructed,and transfected into the colorectal cancer SW620 cells and verified.The effects of LncRNA-ROR overexpression on the function and colony formation of SW620 cells were analyzed.Results:Compared with the normal tissue,the expression level of LncRNA-ROR was low in colorectal cancer tissue(P<0.01),and its expression level was closely related to TNM stage(P<0.05).After transfection,the expression level of LncRNA-ROR increasing in SW620 cells was identified by qRT-PCR,and its high expression led to the decreasing of proliferation function of SW620 cells(P<0.01).Conclusions:The overexpression of LncRNA-ROR can inhibit the proliferation of SW620 cells.

关 键 词：结直肠肿瘤 LncRNA-ROR SW620细胞 增殖 

分 类 号：R735.3[医药卫生—肿瘤]