ARTP-NTG复合诱变选育高产中性蛋白酶菌株  

Screening of High Neutral Protease Producing Strains by ARTP-NTG Compound Mutagenesis

作　　者：钱娟娟 王克芬 宋静静 刘胜利 王兴吉 刘文龙 QIAN Juan-juan;WANG Ke-fen;SONG Jing-jing(Shandong Long Kete Enzyme Co.,Ltd.,Shandong Province Key Laboratory of Enzyme Preparation Fermentation Technology,Linyi,Shandong 276400)

机构地区：[1]山东隆科特酶制剂有限公司山东省酶制剂发酵技术重点实验室

出　　处：《安徽农业科学》2019年第21期5-7,共3页Journal of Anhui Agricultural Sciences

基　　金：国家重点研发计划(2017YFB0308400)

摘　　要：以枯草芽孢杆菌A-06为出发菌株,利用ARTP-亚硝基胍(NTG)复合诱变方法对该菌株进行遗传改造。经过平板初筛,摇瓶复筛,最终筛选出一株酶活提高明显、遗传稳定的菌株N-36。其摇瓶发酵酶活达29 500 U/mL,较出发菌株酶活提高90%左右。The original strain of Bacillus subtilis A-06 was modified in genetic characteristics by ARTP-NTG compound mutagenesis.One high neutral protease-yielding strain N-36 was screened from the mutants by slab screening and shake flask rescreening,which the enzyme activity increased obviously and had good genetic stability.The enzyme activity of the shake flask-fermentation reached 29 500 U/mL,which was 90%higher than that of the original strain.

关 键 词：中性蛋白酶 ARTP NTG 复合诱变 筛选 

分 类 号：Q933[生物学—微生物学]