Ⅱ型猪链球菌的分离鉴定及药敏试验  

Isolation,Identification and Drug Sensitivity Test of Streptococcus suis Type 2

作　　者：刘运镇[1] 桂文龙[1] 温思怡 谢昕芳 LIU Yun-zhen;GUI Wen-long;WEN Si-yi(Jiangsu Agri-animal Vocational College,Taizhou,Jiangsu 225300)

机构地区：[1]江苏农牧科技职业学院

出　　处：《安徽农业科学》2019年第21期102-104,共3页Journal of Anhui Agricultural Sciences

基　　金：江苏省大学生创新创业训练计划项目;常州金牧药业有限公司产学研合作项目(00010114011)

摘　　要：2018年8月,江苏省苏中地区某猪场出现疑似感染猪链球菌,病理剖检可见发病猪表现为脑膜、肺脏、肾脏、淋巴结出血肿胀,关节腔有纤维素性渗出物等病理变化,取鼻、肺、脑等组织进行细菌鲜血培养基分离培养24 h可见不同的溶血现象;染色镜检可见紫色成对、短链或长链排列的球菌;生化试验对乳糖、海藻糖、七叶苷产酸产气,甘露醇、山梨醇等不反应;经PCR检测最终确诊该猪场为Ⅱ型猪链球菌感染。药敏试验结果表明,该猪场猪链球菌对庆大霉素等常规药物不敏感,而对头孢曲松、氨苄西林具有较强的敏感性。In August of 2018,a pig farm in central area of Jiangsu Province was suspected to be infected with Streptococcus suis.Pathological examination revealed that the pigs were manifested as following:the meninges,lungs,kidneys and lymph nodes were hemorrhagic and swollen,and there were cellulosic exudates in the articular cavity;the tissues of nose,lung and brain were isolated and cultured on bacterial blood culture medium,and different hemolysis phenomena were observed 24 hours later;the purple,paired,short-chain or long-chain cocci were seen by staining microscopy;biochemical experiments showed that it produced acid and gas from lactose,trehalose and aescin.Mannitol and sorbitol did not react;the infection of S.suis type 2 was finally diagnosed by PCR.At the same time,the dug sensitivity test showed that S.suis was not susceptible to routine drugs such as gentamicin,and had strong susceptibility to ceftriaxone and ampicillin.

关 键 词：Ⅱ型链球菌 药敏试验 分离 鉴定 PCR 

分 类 号：S858.28[农业科学—临床兽医学]