桃色顶孢霉获得及其发酵液对马铃薯疮痂病菌的抑制作用  

Obtain of Acremonium persicinum and inhibition of its fermentation on Streptomyces scabies

作　　者：马东梅 李双 台莲梅[1] 刘昕旸 刘振华[1] 孙冬梅[1] MA Dong-mei;LI Shuang;TAI Lian-mei;LIU Xin-yang;LIU Zhen-hua;SUN Dong-mei(Heilongjiang Bayi Agricultural University,Daqing 163319,Helongjiang,China)

机构地区：[1]黑龙江八一农垦大学

出　　处：《湖北农业科学》2019年第20期84-87,共4页Hubei Agricultural Sciences

基　　金：黑龙江省农垦总局重点攻关项目（HNK135-02-08-04）;黑龙江省教育厅产业化前期研发培育项目（1254CGZH32）

摘　　要：从土壤中分离得菌株,利用形态学及18S rDNA测序确定所得菌株为桃色顶孢霉(Acremonium persicinum)。测定该菌株发酵液不同浓度、不同酸碱度、高温处理和较长保存时间条件下,对马铃薯疮痂病菌(Streptomyces scabies)的抑菌作用。结果表明,不同浓度的发酵液对疮痂病菌均有明显抑制作用,浓度为75%时抑菌圈半径最大,为17.1 mm;发酵液经121℃20 min处理后,抑菌作用与不处理的相近;室温保存9个月的发酵液仍有抑菌效果,但抑菌活性比保存1个月下降了26.43%;发酵液pH调整为3~8时,抑菌效果良好,且中性、偏碱性比酸性发酵液抑菌效果好。该菌株发酵液有较好抑菌效果且稳定性良好,有较好的应用前景。Antagonistic fungi was obtained by soil separation,and the strain was identified as Acremonium persicinum by morphology and 18S rDNA sequencing.The effects of its fermentation on Streptomyces scabies were determined by changing the concentration,pH,temperature and preservation time.Results showed that the inhibition ability of different concentrations were obviously,and the maximum inhibition zone was 17.1 mm of 75%concentration.After the fermentation liquid was treated at 121℃for 20 min,the antibacterial activity was similar to that of the non-treatment.The fermentation liquid kept at room temperature for 9 months still had an antimicrobial effect,but the antibacterial activity decreased by 26.43%,which was lower than one month after preservation.When pH of fermentation liquid was adjusted to 3~8,the inhibition ability was sitll good.Neutral,alkaline fermentation liquid has better antimicrobial effects than acid fermentation liquid.The fermentation liquid of the strain has good antibacterial effect and stability,and has a good application prospect.

关 键 词：马铃薯疮痂病菌(Streptomyces scabies) 桃色顶孢霉(Acremonium persicinum) 发酵液 抑菌效果 

分 类 号：S432.42[农业科学—植物病理学]